Phytochemical, Antioxidant, Antihyaluronidase, Antityrosinase, and Antimicrobial Properties of L. Leaf Extracts.

L. (tobacco) is an important and valuable crop for the cigarette industry. However, cigarette cessation has been encouraged worldwide. Therefore, this study aimed to investigate the potential of leaf extract use in other industries besides cigarette production, especially cosmeceutical industries, which are of interest for increasing the value and widening the applications of . The leaves of var. Virginia and Turkish were extracted by maceration using 95% v/v ethanol or petroleum ether. The extracts were evaluated for their phytochemical compositions, antioxidant capacity, and anti-aging, antimelanogenic, and antimicrobial activities. The phytochemical screening of the extracts revealed terpenoids, steroids, alkaloids, tannins, and carbohydrates in all of the leaf extracts. The total phenolic content was detected to be the highest in the ethanolic extract of Virginia tobacco leaf, which had the most significantly potent antioxidant and antihyaluronidase activity ( < 0.05). On the contrary, the extracts from the Turkish variety demonstrated the most powerful antimicrobial activity against . Thus, ethanolic extracts of var. Virginia are suggested as good natural anti-aging ingredients with potent antioxidant and antihyaluronidase effects, whereas the leaf of var. Turkish is suggested as a good source of natural antimicrobial components, particularly for inhibition. In summary, in addition to the cigarette industry, leaf could be a source of pharmaceutical and cosmeceutical compounds, particularly natural anti-aging and antimicrobial ingredients.