光合作用相关基因融合体的表达在转基因植物和转染原生质体中受细胞类型限制。
Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts.
作者信息
Harkins K R, Jefferson R A, Kavanagh T A, Bevan M W, Galbraith D W
机构信息
Department of Plant Sciences, University of Arizona, Tucson 85721.
出版信息
Proc Natl Acad Sci U S A. 1990 Jan;87(2):816-20. doi: 10.1073/pnas.87.2.816.
We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.
我们分析了嵌合基因在从转基因烟草植物叶片中的光合和非光合组织分离出的原生质体群体中的表达情况，这些原生质体通过荧光激活细胞分选进行分离。由光合作用相关基因的启动子控制的转录基因融合的表达表现出对细胞类型的显著依赖性。当将基因融合体转染到正常（非转基因）烟草叶原生质体中时，这些表达模式得以保留。
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