Rühle Thilo, Reiter Bennet, Leister Dario
Plant Molecular Biology, Department of Biology, Ludwig Maximilian University of Munich, Munich, Germany.
Front Plant Sci. 2018 Jan 30;9:55. doi: 10.3389/fpls.2018.00055. eCollection 2018.
Measurements of chlorophyll fluorescence provide an elegant and non-invasive means of probing the dynamics of photosynthesis. Advances in video imaging of chlorophyll fluorescence have now made it possible to study photosynthesis at all levels from individual cells to entire crop populations. Since the technology delivers quantitative data, is easily scaled up and can be readily combined with other approaches, it has become a powerful phenotyping tool for the identification of factors relevant to photosynthesis. Here, we review genetic chlorophyll fluorescence-based screens of libraries of Arabidopsis and Chlamydomonas mutants, discuss its application to high-throughput phenotyping in quantitative genetics and highlight potential future developments.
叶绿素荧光测量提供了一种巧妙且非侵入性的方法来探究光合作用的动态过程。叶绿素荧光视频成像技术的进步使得从单个细胞到整个作物群体的各个层面研究光合作用成为可能。由于该技术能提供定量数据,易于扩展规模且可轻松与其他方法结合,它已成为用于鉴定与光合作用相关因素的强大表型分析工具。在此,我们综述了基于叶绿素荧光对拟南芥和衣藻突变体文库进行的遗传筛选,讨论了其在数量遗传学高通量表型分析中的应用,并突出了未来潜在的发展方向。