电场介导的DNA转移：人类和小鼠淋巴细胞中的瞬时和稳定基因表达
Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells.
作者信息
Toneguzzo F, Hayday A C, Keating A
出版信息
Mol Cell Biol. 1986 Feb;6(2):703-6. doi: 10.1128/mcb.6.2.703-706.1986.
Abstract
The technique of DNA transfer by electroporation was investigated in an effort to evaluate its utility for the identification of developmentally controlled regulatory sequences. Transient and stable gene expression was detected in a variety of lymphoid cell lines subjected to electroporation. No correlation existed between the levels of chloramphenicol acetyltransferase (acetyl-CoA; chloramphenicol 3-O-acetyltransferase, EC 2.3.1.28) expression and stable transfection frequency. In all lymphoid cell lines tested, the simian virus 40 early region was a better promoter than was the Rous sarcoma virus long terminal repeat.
摘要
为了评估电穿孔法在鉴定发育调控的调控序列中的效用，对通过电穿孔进行DNA转移的技术进行了研究。在多种经电穿孔处理的淋巴细胞系中检测到了瞬时和稳定的基因表达。氯霉素乙酰转移酶（乙酰辅酶A；氯霉素3 - O - 乙酰转移酶，EC 2.3.1.28）的表达水平与稳定转染频率之间不存在相关性。在所有测试的淋巴细胞系中，猿猴病毒40早期区域是比劳氏肉瘤病毒长末端重复序列更好的启动子。
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本文引用的文献
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Electric field mediated gene transfer.电场介导的基因转移。
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Electric field-induced cell-to-cell fusion.电场诱导的细胞间融合。
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Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.筛选表达编码黄嘌呤 - 鸟嘌呤磷酸核糖转移酶的大肠杆菌基因的动物细胞。
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Production of human hybridomas secreting antibodies to measles virus.分泌抗麻疹病毒抗体的人杂交瘤的制备。
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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
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Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes.哺乳动物细胞摄取DNA的机制：利用荧光染料监测外源添加DNA的命运
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Establishment of a near-tetraploid B-cell lymphoma line with duplication of the 8;14 translocation.建立具有8;14易位重复的近四倍体B细胞淋巴瘤细胞系。
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Ia antigen-bearing B cell tumor lines can present protein antigen and alloantigen in a major histocompatibility complex-restricted fashion to antigen-reactive T cells.携带Ia抗原的B细胞肿瘤系能够以主要组织相容性复合体限制的方式将蛋白质抗原和同种异体抗原呈递给抗原反应性T细胞。
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Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.通过电穿孔导入小鼠前B淋巴细胞的人κ免疫球蛋白基因的增强子依赖性表达。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7161-5. doi: 10.1073/pnas.81.22.7161.
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Immunoglobulin gene expression in transformed lymphoid cells.转化淋巴细胞中的免疫球蛋白基因表达。
Proc Natl Acad Sci U S A. 1983 Feb;80(3):825-9. doi: 10.1073/pnas.80.3.825.
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