Raffi Hajamohideen S, Bates James M, Flournoy Dayl J, Kumar Satish
Department of Medicine/Nephrology, University of Oklahoma Health Sciences Center and Veterans Affairs Medical Center, 920 SL Young Blvd, WP 2250, Oklahoma City, OK 73104, USA.
BMC Res Notes. 2012 Sep 26;5:532. doi: 10.1186/1756-0500-5-532.
Urinary catheters are associated, commonly with bacteriuria and frequently with urinary tract infection. Tamm-Horsfall Protein (THP) is urine's most abundant protein and is known to bind to uropathogenic bacteria. The role of THP in the pathogenesis of catheter associated urinary tract infection (CAUTI) is not clear. We examined the role of THP in facilitating bacterial binding to urinary catheters in vivo and in vitro.
Twenty one urinary catheters were obtained from 20 hospitalized patients. THP was eluted from the catheter surface and catheter segments were cultured. Additional studies were performed in vitro on unused silicone and latex catheters to determine the binding of THP, and the effect of THP on the binding of Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa), to the catheter surface.On catheters obtained from patients, the THP deposition was significantly more on culture positive catheters than on culture negative catheters. In the in vitro studies, THP bound to both silicone and latex catheters, and THP enhanced the adherence of E. coli and P. aeruginosa to both types of catheters.
THP binds to urinary catheters and facilitates the binding of uropathogenic bacteria to catheters.
导尿管通常与菌尿症相关，且频繁引发尿路感染。Tamm-Horsfall蛋白（THP）是尿液中含量最丰富的蛋白质，已知其可与尿路致病菌结合。THP在导尿管相关尿路感染（CAUTI）发病机制中的作用尚不清楚。我们研究了THP在体内和体外促进细菌与导尿管结合方面的作用。
从20名住院患者处获取了21根导尿管。从导管表面洗脱THP，并对导管片段进行培养。对未使用的硅胶和乳胶导尿管进行了体外额外研究，以确定THP的结合情况，以及THP对大肠杆菌（E. coli）和铜绿假单胞菌（P. aeruginosa）与导管表面结合的影响。在从患者处获取的导尿管上，培养阳性的导管上THP沉积明显多于培养阴性的导管。在体外研究中，THP与硅胶和乳胶导尿管均有结合，且THP增强了大肠杆菌和铜绿假单胞菌对两种类型导管的黏附。
THP与导尿管结合，并促进尿路致病菌与导尿管的结合。
