School of Stomatology, China Medical University, Shenyang 110002, China.
Colloids Surf B Biointerfaces. 2013 Jan 1;101:343-9. doi: 10.1016/j.colsurfb.2012.06.029. Epub 2012 Jul 3.
The biocompatibility of TiN coated nickel-titanium shape memory alloy (NiTi-SMA) was evaluated to compare with that of the uncoated NiTi-SMA. Based on the orthodontic clinical application, the surface properties and biocompatibility were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), wettability test, mechanical test and in vitro tests including MTT, cell apoptosis and cell adhesion tests. It was observed that the bonding between the substrate and TiN coating is excellent. The roughness and wettability increased as for the TiN coating compared with the uncoated NiTi-SMA. MTT test showed no significant difference between the coated and uncoated NiTi-SMA, however the percentage of early cell apoptosis was significantly higher as for the uncoated NiTi alloy. SEM results showed that TiN coating could enhance the cell attachment, spreading and proliferation on NiTi-SMA. The results indicated that TiN coating bonded with the substrate well and could lead to a better biocompatibility.
氮化钛涂层镍钛形状记忆合金（NiTi-SMA）的生物相容性进行了评估，以与未涂层的 NiTi-SMA 进行比较。基于正畸临床应用，通过扫描电子显微镜（SEM）、X 射线衍射（XRD）、润湿性试验、力学试验和体外试验（包括 MTT、细胞凋亡和细胞黏附试验）对表面性能和生物相容性进行了表征。结果表明，基体与 TiN 涂层之间的结合非常优异。与未涂层的 NiTi-SMA 相比，TiN 涂层的粗糙度和润湿性都有所提高。MTT 试验表明，涂层和未涂层的 NiTi-SMA 之间没有显著差异，但未涂层的 NiTi 合金的早期细胞凋亡率明显更高。SEM 结果表明，TiN 涂层可以促进 NiTi-SMA 上细胞的附着、铺展和增殖。结果表明，TiN 涂层与基体结合良好，可提高生物相容性。
