H H Wills Physics Laboratory, University of Bristol, Tyndall Avenue, Bristol BS8 1TL, UK.
Nanotechnology. 2012 Oct 19;23(41):415601. doi: 10.1088/0957-4484/23/41/415601. Epub 2012 Sep 25.
The synthesis of magnetic, monodisperse nanoparticles has attracted great interest in nanoelectronics and nanomedicine. Here we report the fabrication of pure magnetite nanoparticles, less than ten nanometers in size, using the cage-shaped protein apoferritin (Fe(3)O(4)-ferritin). Crystallizable proteins were obtained through careful successive separation methods, including a magnetic chromatography that enabled the effective separation of proteins, including a Fe(3)O(4) nanoparticle (7.9 ± 0.8 nm), from empty ones. Macroscopic protein crystals allowed the fabrication of three-dimensional arrays of Fe(3)O(4) nanoparticles with interparticle gaps controlled by dehydration, decreasing their magnetic susceptibilities and increasing their blocking temperatures through enhanced dipole-dipole interactions.
磁性、单分散纳米粒子的合成在纳米电子学和纳米医学领域引起了极大的兴趣。在这里，我们报告了使用笼形蛋白脱铁铁蛋白（Fe(3)O(4)-铁蛋白）制备小于 10 纳米的纯磁铁矿纳米粒子。通过仔细的连续分离方法获得可结晶蛋白，包括一种磁色谱法，可有效分离蛋白质，包括 Fe(3)O(4)纳米颗粒（7.9 ± 0.8nm）和空蛋白。宏观蛋白质晶体允许制造具有通过脱水控制的粒子间间隙的 Fe(3)O(4)纳米粒子的三维阵列，通过增强偶极-偶极相互作用降低它们的磁化率并增加它们的阻塞温度。
