Division of Pediatric Infectious Disease, Department of Pediatrics, China Medical University Hospital, China Medical University School of Medicine, Taichung, Taiwan ROC.
Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan ROC.
J Med Microbiol. 2012 Jul;61(Pt 7):990-997. doi: 10.1099/jmm.0.042499-0. Epub 2012 Mar 30.
The molecular characterization of genotype P[6] rotavirus strains collected from children admitted to hospital with acute dehydrating diarrhoea during a 6-year surveillance period in Taiwan is described in this study. In total, three G4P[6] strains, one G5P[6] and one G12P[6] were characterized by sequencing and phylogenetic analysis of the VP4, VP7, VP6 and NSP4 genes. Whilst all four genes of the single Taiwanese G12P[6] strain clustered with the respective genes of globally common human rotavirus strains, the G4 and G5 strains showed remarkable similarities to porcine rotavirus strains and putative porcine-origin human P[19] strains reported previously from Taiwan. The overall proportion of porcine rotavirus-like strains in Taiwan remains around 1 % among hospitalized children; however, the circulation and sporadic transmission of these heterotypic strains from pigs to humans could pose a public-health concern. Therefore, continuation of strain monitoring is needed in the vaccine era to detect any possible vaccine breakthrough events associated with the introduction of such heterologous rotavirus strains.
本研究描述了在台湾为期 6 年的监测期间，从因急性脱水性腹泻住院的儿童中采集的基因型 P[6]轮状病毒毒株的分子特征。总共对 3 株 G4P[6]、1 株 G5P[6]和 1 株 G12P[6]毒株进行了测序和系统进化分析，对 VP4、VP7、VP6 和 NSP4 基因进行了分析。虽然台湾唯一的一株 G12P[6]毒株的所有 4 个基因均与全球常见的人类轮状病毒株的相应基因聚在一起，但 G4 和 G5 株与先前从台湾报告的猪轮状病毒株和推定的猪源人 P[19]株具有显著相似性。在住院儿童中，台湾的猪轮状病毒样株的总体比例仍约为 1%；然而，这些异源株从猪传播到人类的循环和散发性传播可能会引起公共卫生关注。因此，在疫苗时代需要继续进行菌株监测，以检测与引入此类异源轮状病毒株相关的任何可能的疫苗突破事件。
