Zhang Songhua, Moss Steven F
Division of Gastroenterology, Department of Medicine, Rhode Island Hospital/Brown University, Providence, RI, USA.
Methods Mol Biol. 2012;921:89-98. doi: 10.1007/978-1-62703-005-2_12.
Animal models are essential for in vivo analysis of Helicobacter-related diseases. Transgenic mice and Mongolian gerbil models have been the corner stone of present research focusing on both bacterial virulence factors and host response to infection. Establishing a reproducible rodent model of persistent Helicobacter pylori infection that resembles the H. pylori-associated gastritis observed in humans was a considerable challenge until Lee et al. (Gastroenterology 112:1386-1397, 1997) successfully adapted a clinical Cag A- and Vac A-expressing strain for the mouse stomach. This so-called SS1 (Sydney) strain has since been extensively used for H. pylori research; other rodent-adapted Helicobacter strains have subsequently been developed and utilized in wild-type and genetically engineered rodent models. These bacteria include both H. pylori and the larger but related species H. felis (originally isolated from cats). In this chapter we focus mainly on these two Helicobacter strains and review the rodent models that have been employed to investigate how Helicobacter species induce gastric inflammation and disease.
动物模型对于幽门螺杆菌相关疾病的体内分析至关重要。转基因小鼠和蒙古沙鼠模型一直是目前聚焦于细菌毒力因子和宿主对感染反应研究的基石。在Lee等人（《胃肠病学》112:1386 - 1397, 1997）成功将一株表达Cag A和Vac A的临床菌株适配到小鼠胃部之前，建立一种可重现的、类似于人类幽门螺杆菌相关性胃炎的持续性幽门螺杆菌感染啮齿动物模型是一项相当大的挑战。自那以后，这种所谓的SS1（悉尼）菌株被广泛用于幽门螺杆菌研究；随后又开发了其他适应啮齿动物的幽门螺杆菌菌株，并在野生型和基因工程啮齿动物模型中加以利用。这些细菌包括幽门螺杆菌以及更大但相关的猫螺杆菌物种（最初从猫身上分离得到）。在本章中，我们主要聚焦于这两种幽门螺杆菌菌株，并综述已用于研究幽门螺杆菌物种如何诱发胃部炎症和疾病的啮齿动物模型。
