Gautam S, Rajkumar B, Landge S P, Dubey S, Nehete P, Boruah L C
Department of Conservative Dentistry and Endodontics, Nepal Medical College, College of Dental Sciences and Hospital, Jorapti, Kathmandu, Nepal.
Nepal Med Coll J. 2011 Dec;13(4):297-300.
The objective of this study was to assess, in vitro, the effectiveness of several concentrations of Metapex (0.22 gm/ml, 0.022 gm/ml, 0.0022 gm/ml) in the elimination of selected microorganisms. Different concentrations of Metapex were prepared by dissolving it in ethanol (99.9%) Pre-sterilized Whatman paper discs, 6 mm in diameter and soaked with the test solution, were prepared and placed onto the previously seeded agar Petri plates. Plates were incubated aerobically for Enterococcus faecalis (E. Faecalis) and Candida albicans (C. albicans), anaerobically for Bacteroid fragilis (B. fragilis) and Propionibacterium acne respectivally. A zone of inhibition was recorded for each plate and the results were analysed statistically. There was significant reduction in the size of zone of inhibition against P. acne, as the concentration of metapex decreases. At lower concentrations of metapex there was no zone of inhibition observed against E. Faecalis, C. Albicans and B. Fragilis. The result of this study suggested that Metapex is a potent antimicrobial agent at higher concentration.
本研究的目的是在体外评估几种浓度的Metapex（0.22克/毫升、0.022克/毫升、0.0022克/毫升）对选定微生物的清除效果。通过将Metapex溶解于乙醇（99.9%）中来制备不同浓度的Metapex。制备直径为6毫米、预先灭菌并浸泡有测试溶液的Whatman纸圆盘，将其放置在预先接种了菌的琼脂培养皿上。对于粪肠球菌（E. Faecalis）和白色念珠菌（C. albicans），培养皿需有氧培养；对于脆弱拟杆菌（B. fragilis）和痤疮丙酸杆菌，则分别进行厌氧培养。记录每个培养皿的抑菌圈，并对结果进行统计学分析。随着Metapex浓度降低，对痤疮丙酸杆菌的抑菌圈大小显著减小。在较低浓度的Metapex下，未观察到对粪肠球菌、白色念珠菌和脆弱拟杆菌的抑菌圈。本研究结果表明，Metapex在较高浓度时是一种有效的抗菌剂。
