Korea Food Research Institute, Seongnam, Republic of Korea.
Food Chem. 2013 Jan 1;136(1):160-3. doi: 10.1016/j.foodchem.2012.07.111. Epub 2012 Aug 5.
Kiwifruit is one of the most popular fruits worldwide, and it has various biological properties, including antioxidant, anti-allergic, and cardiovascular protective effects. The peel of kiwifruit, which is a by-product of processing, is a good source of flavonoids; however, its bioactivity has not been widely investigated. In this study, we evaluated the hypnotic effects of green (GRPE, Actinidia deliciosa) and gold (GOPE, Actinidia chinensis) kiwifruit peel ethanol extracts and their solvent fractions, and the possible underlying mechanisms. Oral GRPE and GOPE administration (125-1000mg/kg) produced a dose-dependent decrease in sleep latency and an increase in sleep duration in pentobarbital-treated mice. Among three different solvent fractions of GRPE and GOPE, ethyl acetate (EA) fractions had the greatest effect on sleep duration at 250mg/kg. The total flavonoid contents of solvent fractions were proportional to sleep duration. Like diazepam (a GABA(A)-benzodiazepine (BZD) receptor agonist), the hypnotic effects of GRPE, GOPE, and their EA fractions were fully inhibited by flumazenil (a GABA(A)-BZD receptor antagonist). These results suggest that potentiation effects of GRPE and GOPE on pentobarbital-induced sleep in mice may be modulated by a GABAergic mechanism.
奇异果是全球最受欢迎的水果之一，具有多种生物特性，包括抗氧化、抗过敏和心血管保护作用。奇异果的果皮是加工的副产品，是黄酮类化合物的良好来源；然而，其生物活性尚未得到广泛研究。在这项研究中，我们评估了绿奇异果皮（GRPE，Actinidia deliciosa）和金奇异果皮（GOPE，Actinidia chinensis）乙醇提取物及其溶剂级分的催眠作用，以及可能的潜在机制。口服 GRPE 和 GOPE 给药（125-1000mg/kg）可使戊巴比妥处理的小鼠的睡眠潜伏期呈剂量依赖性降低，并增加睡眠时间。在 GRPE 和 GOPE 的三种不同溶剂级分中，乙酸乙酯（EA）级分在 250mg/kg 时对睡眠时间的影响最大。溶剂级分的总黄酮含量与睡眠时间成正比。与地西泮（一种 GABA(A)-苯二氮䓬（BZD）受体激动剂）一样，GRPE、GOPE 及其 EA 级分的催眠作用被氟马西尼（一种 GABA(A)-BZD 受体拮抗剂）完全抑制。这些结果表明，GRPE 和 GOPE 对小鼠戊巴比妥诱导睡眠的增强作用可能是通过 GABA 能机制调节的。
