Parkar Shanthi G, Jobsis Carel M H, Trower Tania M, Cooney Janine M, Hedderley Duncan I, Bentley-Hewitt Kerry L
The New Zealand Institute for Plant and Food Research Limited (Plant & Food Research), Private Bag 11600, Palmerston North 4442, New Zealand.
Plant & Food Research, Private Bag 11600, Hamilton 3240, New Zealand.
Microorganisms. 2020 Oct 14;8(10):1582. doi: 10.3390/microorganisms8101582.
Whole kiwifruit ('Hayward' and 'Zesy002') were examined for their bioaminergic potential after being subjected to in vitro gastrointestinal digestion and colonic fermentation. Controls included the prebiotic inulin and water, a carbohydrate-free vehicle. The dopamine precursor l-dihydroxyphenylalanine (L-DOPA) and the serotonin precursor 5-hydroxytryptophan were increased in the kiwifruit gastrointestinal digesta ('Hayward' > 'Zesy002') in comparison to the water digesta. Fermentation of the digesta with human fecal bacteria for 18 h modulated the concentrations of bioamine metabolites. The most notable were the significant increases in L-DOPA ('Zesy002' > 'Hayward') and γ-aminobutyric acid (GABA) ('Hayward' > 'Zesy002'). Kiwifruit increased spp. and Veillonellaceae (correlating with L-DOPA increase), and spp. (correlating with GABA). The digesta and fermenta were incubated with Caco-2 cells for 3 h followed by gene expression analysis. Effects were seen on genes related to serotonin synthesis/re-uptake/conversion to melatonin, gut tight junction, inflammation and circadian rhythm with different digesta and fermenta from the four treatments. These indicate potential effects of the substrates and the microbially generated organic acid and bioamine metabolites on intestinal functions that have physiological relevance. Further studies are required to confirm the potential bioaminergic effects of gut microbiota-kiwifruit interactions.
对整个奇异果（“海沃德”和“Zesy002”）进行体外胃肠消化和结肠发酵后，检测其生物胺能潜力。对照组包括益生元菊粉和水（一种无碳水化合物载体）。与水消化物相比，奇异果胃肠消化物（“海沃德”>“Zesy002”）中的多巴胺前体L - 二羟基苯丙氨酸（L - DOPA）和血清素前体5 - 羟色氨酸增加。用人类粪便细菌对消化物进行18小时发酵，调节了生物胺代谢物的浓度。最显著的是L - DOPA（“Zesy002”>“海沃德”）和γ-氨基丁酸（GABA）（“海沃德”>“Zesy002”）的显著增加。奇异果增加了 spp. 和韦荣氏菌科（与L - DOPA增加相关），以及 spp.（与GABA相关）。将消化物和发酵物与Caco - 2细胞孵育3小时，然后进行基因表达分析。在来自四种处理的不同消化物和发酵物中，观察到对与血清素合成/再摄取/转化为褪黑素、肠道紧密连接、炎症和昼夜节律相关基因的影响。这些表明底物以及微生物产生的有机酸和生物胺代谢物对具有生理相关性的肠道功能具有潜在影响。需要进一步研究以证实肠道微生物群与奇异果相互作用的潜在生物胺能效应。
