School of Science and Health, University of Western Sydney, Hawkesbury, New South Wales, Australia.
Microsc Res Tech. 2012 Dec;75(12):1717-22. doi: 10.1002/jemt.22121. Epub 2012 Sep 28.
Analysis of the cellular distributions of coenzymes including NADH may aid in understanding a cells metabolic status. We altered serum concentration (0, 2, and 10%) to induce living myoblast cells to undergo the early stages of differentiation. Through microscopy and phasor-FLIM, we spatially mapped and identified variations in the distribution of free and bound NADH. Undifferentiated cells displayed abundant free NADH within the nucleus along with specific regions of more bound NADH. Complete serum starvation dramatically increased the fraction of bound NADH in the nucleus, indicating heightened requirement for transcriptional processes. In comparison, cells exposed to 2% serum exhibited intermediate free nuclear NADH fraction. Overall our results suggest an order of events in which a cell metabolic status alters significantly during the early stages of serum induced differentiation.
分析包括 NADH 在内的辅酶的细胞分布可能有助于了解细胞的代谢状态。我们改变血清浓度（0%、2%和 10%），诱导活肌细胞经历早期分化阶段。通过显微镜和相因子-FLIM，我们对自由态和结合态 NADH 的分布进行了空间映射和识别。未分化细胞在核内显示出丰富的游离 NADH，同时还有特定区域的结合态 NADH。完全血清饥饿显著增加了核内结合态 NADH 的分数，表明转录过程的需求增加。相比之下，暴露于 2%血清的细胞显示出中间的核内游离 NADH 分数。总的来说，我们的结果表明，在血清诱导的分化早期，细胞代谢状态会发生显著变化。
