Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology-AIST, Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Acta Biomater. 2013 Feb;9(2):5080-7. doi: 10.1016/j.actbio.2012.09.027. Epub 2012 Sep 27.
To characterize the differences in the receptor-binding specificities of human and avian influenza viruses with glycan chains, the authors performed binding analyses using an evanescent field-coupled waveguide-mode biosensor. The experiments were performed on intact viruses and hemagglutinin proteins, using gold-nanoparticle-conjugated Neu5Acα2,6Gal and Neu5Acα2,3Gal glycan chains. Several influenza viruses belonging to subtypes H3N2 (A/Udorn/307/1972, A/Shandong/9/1993, A/Kiev/301/1994, A/Panama/2007/1999, A/Wisconsin/67/2005 and A/Brisbane/10/2007), H1N1 (A/Brisbane/59/2007 and A/California/07/2009) and H5N1 (A/chicken/India/NIV33487/2006) were used. High levels of glycan-based discrimination were observed with the H3N2 strain A/Brisbane/10/2007 due to its specificity with Neu5Acα2,6Gal, but not with Neu5Acα2,3Gal. Possible amino acid residues responsible for the discrimination of human and avian influenza viruses are discussed. These types of sensor-based discriminatory analyses would be very useful for distinguishing between influenza pandemics, especially during the transition and overlapping periods of human and avian influenza viruses with evolutionary changes.
为了研究人类和禽类流感病毒与糖链的受体结合特异性的差异，作者使用倏逝场耦合波导模式生物传感器进行了结合分析。实验在完整病毒和血凝素蛋白上进行，使用了金纳米粒子偶联的 Neu5Acα2,6Gal 和 Neu5Acα2,3Gal 糖链。研究使用了几种属于 H3N2（A/Udorn/307/1972、A/Shandong/9/1993、A/Kiev/301/1994、A/Panama/2007/1999、A/Wisconsin/67/2005 和 A/Brisbane/10/2007）、H1N1（A/Brisbane/59/2007 和 A/California/07/2009）和 H5N1（A/chicken/India/NIV33487/2006）亚型的流感病毒。由于其对 Neu5Acα2,6Gal 的特异性，H3N2 株 A/Brisbane/10/2007 表现出高水平的基于聚糖的区分，而对 Neu5Acα2,3Gal 则没有。讨论了可能负责区分人类和禽类流感病毒的氨基酸残基。这些基于传感器的区分分析对于区分流感大流行非常有用，尤其是在人类和禽类流感病毒与进化变化相关的过渡和重叠期间。
