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基于弹性光散射光谱的刚性内窥镜偏振多光谱成像。

Polarized multispectral imaging in a rigid endoscope based on elastic light scattering spectroscopy.

作者信息

Qi Ji, Barrière Clement, Wood Tobias C, Elson Daniel S

机构信息

Hamlyn Centre for Robotic Surgery, Institute of Global Health Innovation, Imperial College London, Exhibition Road, London SW7 2AZ, UK ; Department of Surgery and Cancer, Imperial College London, Exhibition Road, London SW7 2AZ, UK.

出版信息

Biomed Opt Express. 2012 Sep 1;3(9):2087-99. doi: 10.1364/BOE.3.002087. Epub 2012 Aug 13.

DOI:10.1364/BOE.3.002087
PMID:23024903
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3447551/
Abstract

Elastic light scattering spectroscopy (LSS) is widely utilized to investigate cellular structures in cultured cells and various tissues. However, few imaging systems, especially endoscopic imaging systems, can implement LSS. It is the aim of this work to create a polarized multispectral imaging system based around a rigid endoscope to detect micrometer sized particles using LSS. The instrument first validated with different sized mono-disperse polystyrene microspheres, then an image is reconstructed based on LSS which shows the differentiation of different sized microspheres. Finally a preliminary experiment is conducted to demonstrate its capability to discriminate different types of cells.

摘要

弹性光散射光谱法（LSS）被广泛用于研究培养细胞和各种组织中的细胞结构。然而，很少有成像系统，尤其是内窥镜成像系统能够实现LSS。这项工作的目的是创建一个基于刚性内窥镜的偏振多光谱成像系统，以利用LSS检测微米级大小的颗粒。该仪器首先用不同大小的单分散聚苯乙烯微球进行验证，然后基于LSS重建图像，该图像显示了不同大小微球的差异。最后进行了一项初步实验，以证明其区分不同类型细胞的能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/e2a9a828e2ae/boe-3-9-2087-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/f26926972843/boe-3-9-2087-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/9bc9ecca9b2c/boe-3-9-2087-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/567640d46362/boe-3-9-2087-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/a2b0efa6ff76/boe-3-9-2087-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/78a2a8c7e0cd/boe-3-9-2087-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/3e4bb716ce03/boe-3-9-2087-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/db6c074f1f07/boe-3-9-2087-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/ffe91655670a/boe-3-9-2087-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/8d8070dea8da/boe-3-9-2087-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/e2a9a828e2ae/boe-3-9-2087-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/f26926972843/boe-3-9-2087-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/9bc9ecca9b2c/boe-3-9-2087-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/567640d46362/boe-3-9-2087-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/a2b0efa6ff76/boe-3-9-2087-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/78a2a8c7e0cd/boe-3-9-2087-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/3e4bb716ce03/boe-3-9-2087-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/db6c074f1f07/boe-3-9-2087-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/ffe91655670a/boe-3-9-2087-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/8d8070dea8da/boe-3-9-2087-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/919f/3447551/e2a9a828e2ae/boe-3-9-2087-g010.jpg

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