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两株临床分离的光滑假丝酵母菌对两性霉素 B 的敏感性降低，均携带有 ERG2 基因突变。

Two clinical isolates of Candida glabrata exhibiting reduced sensitivity to amphotericin B both harbor mutations in ERG2.

机构信息

Institute of Life Science, Swansea University, Swansea, Wales, UK.

出版信息

Antimicrob Agents Chemother. 2012 Dec;56(12):6417-21. doi: 10.1128/AAC.01145-12. Epub 2012 Oct 1.

DOI:10.1128/AAC.01145-12
PMID:23027188
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3497184/
Abstract

Two novel isolates of Candida glabrata exhibiting reduced sensitivity to amphotericin B (MIC, 8 μg ml(-1)) were found to be ERG2 mutants, wherein Δ(8)-sterol intermediates comprised >90% of the total cellular sterol fraction. Both harbored an alteration at Thr(121) in ERG2; the corresponding residue (Thr(119)) in Saccharomyces cerevisiae is essential for sterol Δ8-Δ7 isomerization. This constitutes the first report of C. glabrata harboring mutations in ERG2 and exhibiting reduced sensitivity to amphotericin B.

摘要

发现两株新型近平滑假丝酵母（Candida glabrata）分离株对两性霉素 B 的敏感性降低（MIC，8μg/ml），它们是 ERG2 突变体，其中 Δ(8)-固醇中间产物占总细胞固醇部分的 >90%。这两个分离株都在 ERG2 的 Thr(121)处发生了改变；酿酒酵母（Saccharomyces cerevisiae）中相应的残基（Thr(119)）对于固醇 Δ8-Δ7 异构化是必需的。这是首例报道的携带 ERG2 突变并对两性霉素 B 敏感性降低的近平滑假丝酵母。

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