开发和鉴定 110 个铁皮石斛 EST-SSR 标记。
Development and characterization of 110 novel EST-SSR markers for Dendrobium officinale (Orchidaceae).
机构信息
Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 310018, People's Republic of China.
出版信息
Am J Bot. 2012 Oct;99(10):e415-20. doi: 10.3732/ajb.1200132. Epub 2012 Oct 1.
PREMISE OF THE STUDY
Expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers were developed in Dendrobium officinale by screening a cDNA library. The loci were verified by sequencing and explored for polymorphism among 19 genotypes and transferability among 30 other distantly related Dendrobium species. •
METHODS AND RESULTS
One hundred ten EST-SSRs were developed, and a set of 20 amplified two to six nucleotide repeats with a mean number of 2.5 alleles per locus and with an observed heterozygosity and polymorphism information content per locus ranging from 0.3463 to 0.9003 and 0.0997 to 0.6537 in 19 D. officinale genotypes, respectively. Furthermore, 92 of these markers have cross-taxa transferability, ranging from 6.45% to 100% among 30 other distantly related Dendrobium species. •
CONCLUSIONS
The developed markers have potential for application in germplasm appraisal, genetic diversity study, genetic mapping, and molecular breeding in D. officinale and other congeneric species.
研究前提
通过筛选 cDNA 文库，从铁皮石斛中开发出基于表达序列标签 (EST) 的简单重复序列 (SSR) 标记。通过测序验证了这些基因座，并在 19 个基因型中探索了多态性，在 30 个其他亲缘关系较远的铁皮石斛属物种中探索了可转移性。
方法和结果
共开发了 110 个 EST-SSR，其中 20 个扩增了 2 到 6 个核苷酸重复，每个基因座平均有 2.5 个等位基因，每个基因座的观测杂合度和多态性信息含量分别为 0.3463 至 0.9003 和 0.0997 至 0.6537，在 19 个铁皮石斛基因型中。此外，这些标记中有 92 个具有跨分类群的可转移性，在 30 个其他亲缘关系较远的铁皮石斛属物种中的转移率为 6.45%至 100%。
结论
所开发的标记可用于铁皮石斛及其同属其他物种的种质评估、遗传多样性研究、遗传图谱构建和分子育种。
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