通过二维凝胶电泳对高分子量RNA进行“指纹图谱”分析：应用于脊髓灰质炎病毒RNA
"Fingerprinting" high molecular weight RNA by two-dimensional gel electrophoresis: application to poliovirus RNA.
作者信息
Lee Y F, Wimmer E
出版信息
Nucleic Acids Res. 1976 Jul;3(7):1647-58. doi: 10.1093/nar/3.7.1647.
Abstract
Conditions are described under which complete RNase T1 digests of high molecular weight RNA can be separated into numerous components by two-dimensional gel electrophoresis. Small and large oligonucleotides (n = 1 - 2c0) can be resolved without losses. The procedure yields fingerprints which are diagnostic for a particular species of RNA and an index of its purity as will be shown for the genomes of poliovirus type 1 and 2.
摘要
本文描述了在何种条件下，通过二维凝胶电泳可将高分子量RNA的完全核糖核酸酶T1消化产物分离成众多组分。大小寡核苷酸（n = 1 - 20）能够无损分离。该方法产生的指纹图谱可用于诊断特定种类的RNA及其纯度指标，如脊髓灰质炎病毒1型和2型基因组所示。
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