Wysocka M, Hackett C J
Wistar Institute, Philadelphia, Pennsylvania 19104.
J Virol. 1990 Mar;64(3):1028-32. doi: 10.1128/JVI.64.3.1028-1032.1990.
Class I major histocompatibility complex-restricted cytotoxic T lymphocytes (CTL) that recognize the neuraminidase (NA) glycoprotein of subtype N1 influenza A viruses have been demonstrated in BALB/c mice. Responses to NA were obtained only in protocols that use two in vivo inoculations of virus, including a recombinant vaccinia virus containing the NA of subtype N1 influenza virus (NA-VAC) to prime or boost. Restimulation in vitro was also required for CTL recognition of NA and strongly depended on the specific N1 virus used. Influenza viruses A/Puerto Rico/8/34 (H1N1), A/CAM/46 (H1N1), J1 (H3N1), and JAP/BEL (H2N1), but not A/Bellamy (H1N1) or MEM/BEL (H3N1) virus, were able to stimulate NA-specific memory T cells in vitro. Single or double in vivo inoculation of any of the N1 viruses or a single injection of NA-VAC failed to elicit restimulatable NA-specific CTL. Lysis of NA-VAC-infected cells at low effector/target ratios was comparable to that observed toward other influenza virus proteins known to be major targets of CTL in BALB/c mice, indicating that antigenic determinants of the subtype N1 NA molecule can be efficiently presented in the context of major histocompatibility complex class I.
在BALB/c小鼠中已证实存在识别甲型N1流感病毒神经氨酸酶（NA）糖蛋白的I类主要组织相容性复合体限制性细胞毒性T淋巴细胞（CTL）。仅在使用两次病毒体内接种的方案中才能获得对NA的反应，其中包括使用含有N1亚型流感病毒NA的重组痘苗病毒（NA-VAC）进行初次免疫或加强免疫。体外再刺激对于CTL识别NA也是必需的，并且强烈依赖于所使用的特定N1病毒。甲型流感病毒A/波多黎各/8/34（H1N1）、A/柬埔寨/46（H1N1）、J1（H3N1）和日本/比利时（H2N1），但不是A/贝拉米（H1N1）或MEM/比利时（H3N1）病毒，能够在体外刺激NA特异性记忆T细胞。对任何一种N1病毒进行单次或两次体内接种或单次注射NA-VAC均未能引发可再刺激的NA特异性CTL。在低效应细胞/靶细胞比例下，NA-VAC感染细胞的裂解与在BALB/c小鼠中已知为CTL主要靶标的其他流感病毒蛋白的裂解情况相当，这表明N1亚型NA分子的抗原决定簇可以在I类主要组织相容性复合体的背景下有效呈递。
