Li X L, Moudgil T, Vinters H V, Ho D D
Department of Medicine, Cedars-Sinai Medical Center and UCLA School of Medicine 90048.
J Virol. 1990 Mar;64(3):1383-7. doi: 10.1128/JVI.64.3.1383-1387.1990.
One neuronal cell line (SK-N-MC) was found to be susceptible to productive infection by multiple isolates of the human immunodeficiency virus type 1 (HIV-1). Characterization of SK-N-MC cells showed that these cells are neuroectodermal in origin in that they express dopamine hydroxylase, catecholamines, neuron-specific enolase, and neurofilaments. Despite their susceptibility to HIV-1 infection, SK-N-MC cells had no detectable CD4 and this infection was not blocked by anti-CD4 monoclonal antibodies (OKT4A, Leu3A) or recombinant soluble CD4. These experiments demonstrated that certain cells of neuroectodermal origin are susceptible to infection in vitro by HIV-1 via a CD4-independent mechanism.
发现一种神经元细胞系（SK-N-MC）易受多种1型人类免疫缺陷病毒（HIV-1）分离株的增殖性感染。对SK-N-MC细胞的特性分析表明，这些细胞起源于神经外胚层，因为它们表达多巴胺羟化酶、儿茶酚胺、神经元特异性烯醇化酶和神经丝。尽管SK-N-MC细胞易受HIV-1感染，但未检测到CD4，且这种感染不受抗CD4单克隆抗体（OKT4A、Leu3A）或重组可溶性CD4的阻断。这些实验表明，某些神经外胚层起源的细胞在体外可通过不依赖CD4的机制受到HIV-1感染。
