Howard Hughes Medical Institute, Boston University, Boston, Massachusetts, USA.
Nat Methods. 2012 Nov;9(11):1077-80. doi: 10.1038/nmeth.2205. Epub 2012 Oct 7.
We present a methodology for the design, construction and modification of synthetic gene networks. This method emphasizes post-assembly modification of constructs based on network behavior, thus facilitating iterative design strategies and rapid tuning and repurposing of gene networks. The ease of post-construction modification afforded by this approach and the ever-increasing repository of components within the framework will help accelerate the development of functional genetic circuits for synthetic biology.
我们提出了一种用于设计、构建和修改合成基因网络的方法。这种方法强调基于网络行为对构建体进行后组装修改，从而促进迭代设计策略以及基因网络的快速调整和重新设计。这种方法提供的后构建修改的简便性以及在该框架内不断增加的组件库将有助于加速合成生物学中功能性遗传电路的发展。
