层粘连蛋白γ1 的表达与脑膜瘤分级、复发和无进展生存率的关系。

Association between laminin γ1 expression and meningioma grade, recurrence, and progression-free survival.

机构信息

Department of Emergency Medicine, Shanghai Sixth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200233, China.

出版信息

Acta Neurochir (Wien). 2013 Jan;155(1):165-71. doi: 10.1007/s00701-012-1512-0. Epub 2012 Oct 5.

DOI:10.1007/s00701-012-1512-0

PMID:23053286

Abstract

BACKGROUND

Laminins are central components of basement membranes and play important roles in cell adhesion, proliferation, and migration. However, the role of laminins in tumor progression has not been thoroughly investigated in meningiomas.

OBJECTIVE

The aim of the present study is to evaluate the expression of laminin γ1 in various grades of meningiomas in Chinese patients.

METHODS

In the current study, clinical and pathological data for 32 meningioma patients with various tumor grades were collected. The expression of laminin γ1 in each tumor was assessed by using quantitative real-time polymerase chain reaction (qPCR), Western blot and immunohistochemical analysis and was correlated with the meningioma grade, tumor recurrence and patient survival. Patient prognoses were attained and the progression-free survival was calculated based on the Kaplan-Meier method. A two-sided probability cutoff of 0.05 was chosen for statistical significance.

RESULTS

A total of 32 meningioma patients with various pathological subtypes (WHO grade I: 13, grade II: 10 and grade III: 9) were enrolled in this study. The qPCR results showed that laminin γ1 mRNA expression was significantly higher in grade III meningiomas than in grade I meningiomas (p < 0.05), although there was no significant difference in laminin γ1 expression between grade II and grade I meningiomas (p > 0.05). Western blot and immunohistochemistry analysis confirmed that the expression of laminin γ1 protein was relatively higher in grade III meningiomas when compared with grade I meningiomas. Higher levels of laminin γ1 expression in meningiomas are associated with a significantly shorter tumor recurrence time (p < 0.05) and a decreased patient survival time (p < 0.05).

CONCLUSIONS

Our results suggest that laminin γ1 is associated with meningioma grades and could play a role in enhancing tumor invasion. Laminin γ1 could be used as a predictor for meningioma recurrence and patient survival. Furthermore, laminin γ1 may represent a druggable molecular target for future therapies for tumors that overexpress this marker.

摘要

背景

层粘连蛋白是基底膜的重要组成部分，在细胞黏附、增殖和迁移中发挥重要作用。然而，层粘连蛋白在脑膜瘤中的作用在肿瘤进展中尚未得到充分研究。

目的

本研究旨在评估层粘连蛋白γ1 在不同级别中国脑膜瘤患者中的表达。

方法

本研究收集了 32 例不同肿瘤级别的脑膜瘤患者的临床和病理资料。通过实时定量聚合酶链反应(qPCR)、Western blot 和免疫组织化学分析评估每个肿瘤中层粘连蛋白γ1 的表达，并与脑膜瘤分级、肿瘤复发和患者生存相关。根据 Kaplan-Meier 方法获得患者预后并计算无进展生存期。选择双侧概率截止值 0.05 作为统计学意义的标准。

结果

本研究共纳入 32 例具有不同病理亚型的脑膜瘤患者（WHO 分级 I：13 例，分级 II：10 例，分级 III：9 例）。qPCR 结果显示，III 级脑膜瘤中层粘连蛋白γ1 mRNA 的表达明显高于 I 级脑膜瘤（p<0.05），而 II 级脑膜瘤中层粘连蛋白γ1 的表达与 I 级脑膜瘤无显著差异（p>0.05）。Western blot 和免疫组织化学分析证实，与 I 级脑膜瘤相比，III 级脑膜瘤中层粘连蛋白γ1 蛋白的表达相对较高。脑膜瘤中较高水平的层粘连蛋白γ1 表达与肿瘤复发时间明显缩短（p<0.05）和患者生存时间缩短（p<0.05）相关。