Institute of Biomedical Engineering, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical College, 270 Xueyuan Road, Wenzhou, 325027, China.
J Mater Sci Mater Med. 2012 Dec;23(12):2857-65. doi: 10.1007/s10856-012-4757-5. Epub 2012 Oct 4.
The development of non-cytotoxic hydrogels that can allow for the controlled release of molecules has important clinical and therapeutic applications. In this paper, we developed a series of in situ hydrogels by combining N,O-carboxymethyl chitosan and oxidized alginate without additional crosslinking agents. The rheological properties of these hydrogels as well as their gelling time, swelling ratio, and in vitro degradation behavior were investigated. We observed that although gelation was rapid at physiological temperature, it was even faster in the presence of higher oxidization degree of alginate. In vitro cytotoxicity study showed that the developed hydrogels were not cytotoxic after 24 h of culturing with NIH-3T3 cells. Additionally, bovine serum albumin was released from the hydrogels initially by diffusion at early stages followed by a degradation-dependent mechanism at later stages. In conclusion, the developed hydrogel might have potential application in the drug delivery system and tissue engineering.
开发无细胞毒性的水凝胶，使其能够控制分子的释放，具有重要的临床和治疗应用。在本文中，我们通过将 N,O-羧甲基壳聚糖和氧化海藻酸钠结合在一起，而无需额外的交联剂，开发了一系列原位水凝胶。研究了这些水凝胶的流变性质以及它们的胶凝时间、溶胀比和体外降解行为。我们观察到，尽管在生理温度下凝胶化迅速，但在海藻酸钠氧化程度较高的情况下，凝胶化甚至更快。体外细胞毒性研究表明，与 NIH-3T3 细胞共培养 24 小时后，所开发的水凝胶没有细胞毒性。此外，牛血清白蛋白最初通过扩散从水凝胶中释放，随后在后期通过降解依赖的机制释放。总之，所开发的水凝胶可能在药物输送系统和组织工程中有潜在的应用。
