Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi
Mediators Inflamm. 2012;2012:493043. doi: 10.1155/2012/493043. Epub 2012 Sep 29.
The aim of this study was to determine the levels of the angiogenic and fibrogenic factors osteopontin (OPN), high-mobility group box-1 (HMGB1), and connective tissue growth factor (CTGF) and the antiangiogenic and antifibrogenic pigment epithelium-derived factor (PEDF) in the vitreous fluid from patients with proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR), and rhegmatogenous retinal detachment with no PVR (RD). Vitreous samples from 48 PDR, 17 PVR and 30 RD patients were studied by enzyme-linked immunosorbent assay. OPN, HMGB1, CTGF, and PEDF levels were significantly higher in PDR patients than in RD patients (P < 0.001; 0.002; <0.001; <0.001, resp.). CTGF and PEDF levels were significantly higher in PVR patients than in RD patients (P < 0.001; 0.004, resp.). Exploratory logistic regression analysis identified significant associations between PDR and high levels of HMGB1, CTGF and PEDF, between PDR with active neovascularization and high levels of CTGF and PEDF, and between PDR with traction retinal detachment and high levels of HMGB1. In patients with PDR, there were significant correlations between the levels of PEDF and the levels of OPN (r = 0.544, P = 0.001), HMGB1 (r = 0.719, P < 0.001), and CTGF (r = 0.715, P < 0.001). In patients with PVR, there were significant correlations between the levels of OPN and the levels of HMGB1 (r = 0.484, P = 0.049) and PEDF (r = 0.559, P = 0.02). Our findings suggest that OPN, HMGB1, and CTGF contribute to the pathogenesis of proliferative vitreoretinal disorders and that increased levels of PEDF may be a response to counterbalance the activity of angiogenic and fibrogenic factors in PDR and PVR.
本研究旨在确定骨桥蛋白（OPN）、高迁移率族蛋白 1（HMGB1）和结缔组织生长因子（CTGF）等血管生成和纤维生成因子以及抗血管生成和抗纤维生成的色素上皮衍生因子（PEDF）在增生性糖尿病视网膜病变（PDR）、增生性玻璃体视网膜病变（PVR）和无 PVR 的孔源性视网膜脱离（RD）患者玻璃体中的水平。通过酶联免疫吸附试验研究了 48 例 PDR、17 例 PVR 和 30 例 RD 患者的玻璃体样本。OPN、HMGB1、CTGF 和 PEDF 水平在 PDR 患者中显著高于 RD 患者（P<0.001；0.002；<0.001；<0.001，分别）。CTGF 和 PEDF 水平在 PVR 患者中显著高于 RD 患者（P<0.001；0.004，分别）。探索性逻辑回归分析确定了 PDR 与高水平 HMGB1、CTGF 和 PEDF 之间、PDR 与活跃新生血管形成与高水平 CTGF 和 PEDF 之间、PDR 与牵引性视网膜脱离与高水平 HMGB1 之间的显著关联。在 PDR 患者中，PEDF 水平与 OPN（r=0.544，P=0.001）、HMGB1（r=0.719，P<0.001）和 CTGF（r=0.715，P<0.001）水平之间存在显著相关性。在 PVR 患者中，OPN 水平与 HMGB1（r=0.484，P=0.049）和 PEDF（r=0.559，P=0.02）水平之间存在显著相关性。我们的研究结果表明，OPN、HMGB1 和 CTGF 有助于增生性玻璃体视网膜疾病的发病机制，PEDF 水平升高可能是对 PDR 和 PVR 中血管生成和纤维生成因子活性的一种平衡反应。
