Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, Doornfontein, South Africa.
Diabetes Technol Ther. 2012 Dec;14(12):1110-7. doi: 10.1089/dia.2012.0125. Epub 2012 Oct 11.
Collagen type I (Col-I) is a major component of the extracellular matrix and is important in wound healing processes. Several studies have shown that low-intensity laser irradiation (LILI) biostimulates Col-I synthesis both in vitro and in vivo. This study aimed to determine if LILI affects collagen production and related cellular responses in an in vitro diabetic wounded fibroblast model.
This study was performed on isolated human skin fibroblasts. Different cell models (normal and diabetic wounded) were used. Cells were irradiated with 5 J/cm(2) at a wavelength of 660 nm and incubated for 48 or 72 h. Nonirradiated cells (0 J/cm(2)) were used as controls. Cellular viability (Trypan blue exclusion test), morphology (bright-field microscopy), proliferation [VisionBlue™ quick cell proliferation assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay], and Col-I (enzyme-linked immunoabsorbent assay) were assessed.
Diabetic wounded cells irradiated with 5 J/cm(2) at 660 nm showed a significant increase in cell migration, viability, proliferation, and collagen content.
This study shows that LILI stimulates Col-I synthesis in diabetic wound healing in vitro at 660 nm.
I 型胶原（Col-I）是细胞外基质的主要成分，在伤口愈合过程中非常重要。多项研究表明，低强度激光辐射（LILI）在体外和体内均能促进 Col-I 的合成。本研究旨在确定 LILI 是否会影响体外糖尿病创伤成纤维细胞模型中的胶原产生和相关细胞反应。
本研究在分离的人皮肤成纤维细胞上进行。使用了不同的细胞模型（正常和糖尿病创伤）。细胞用波长为 660nm 的 5J/cm² 的激光照射，孵育 48 或 72 小时。未照射的细胞（0J/cm²）用作对照。通过台盼蓝排除试验评估细胞活力，通过明场显微镜评估细胞形态，通过 VisionBlue™快速细胞增殖测定和 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐测定评估增殖，通过酶联免疫吸附测定评估 Col-I。
660nm 处 5J/cm² 激光照射的糖尿病创伤细胞显示出细胞迁移、活力、增殖和胶原含量的显著增加。
本研究表明，660nm 处的 LILI 可刺激糖尿病创面愈合过程中的 Col-I 合成。
