Dhilip Kumar Sathish Sundar, Nadene Houreld Nicolette, Abrahamse Heidi
Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Johannesburg, 2028, South Africa.
Sci Rep. 2025 May 6;15(1):15793. doi: 10.1038/s41598-025-99400-2.
In nanomedicine, gold nanoparticles (GNPs) are based on metal-based nanomaterials and have attracted considerable attention from researchers for their use in drug delivery, including wound healing. This study examined the use of Gum tragacanth (GT) for producing gold nanoparticles (GNPs) through the green synthesis method (GT-GNPs). The antibacterial activity of GT-GNPs against pathogenic bacterial strains, as well as assess the wound healing potential of GT-GNPs combined with photobiomodulation (PBM) on normal and diabetic wound models (in vitro). In physicochemical characterization results, we found that the synthesized nanoparticles are 10-20 nm with an average of 12 ± 2 nm in size, and smooth surface and confirmed the presence of GT on the GT-GNPs. It also possessed optimal antibacterial activity and was confirmed through flow cytometry studies. Furthermore, we examined the combined effect of GT-GNPs and PBM against normal and diabetic wound models in WS1 (Humal Fibroblast cells), including cell morphology, percentage of wound closure, nuclear, and filamentous (F)-actin morphology. The combined effects of GT-GNPs and PBM effectively achieved progressive wound closure. In summary, this study has demonstrated that treating GT-GNPs has no adverse effects on both normal and diabetic wound healing processes.
在纳米医学中，金纳米颗粒（GNPs）是基于金属的纳米材料，因其在药物递送（包括伤口愈合）中的应用而备受研究人员关注。本研究考察了通过绿色合成法利用刺梧桐胶（GT）制备金纳米颗粒（GT-GNPs）。研究了GT-GNPs对致病细菌菌株的抗菌活性，并评估了GT-GNPs联合光生物调节（PBM）对正常和糖尿病伤口模型（体外）的伤口愈合潜力。在物理化学表征结果中，我们发现合成的纳米颗粒大小为10-20nm，平均大小为12±2nm，表面光滑，并证实了GT-GNPs上存在GT。它还具有最佳的抗菌活性，并通过流式细胞术研究得到证实。此外，我们研究了GT-GNPs和PBM对WS1（人成纤维细胞）中正常和糖尿病伤口模型的联合作用，包括细胞形态、伤口闭合百分比、细胞核和丝状（F）-肌动蛋白形态。GT-GNPs和PBM的联合作用有效地实现了伤口的逐步闭合。总之，本研究表明，治疗性GT-GNPs对正常和糖尿病伤口愈合过程均无不良影响。
