Department of Pharmacology, School of Medicine, Shenzhen University, Shenzhen 518060, China.
J Ethnopharmacol. 2012 Dec 18;144(3):584-91. doi: 10.1016/j.jep.2012.09.047. Epub 2012 Oct 11.
Ligustrum purpurascens, named as "Ku ding cha", has been used as a kind of functional tea in southern China for about two thousand years, which has the effects on diuresis, anti-hypertension, weight-loss and anti-inflammation.
This study was aimed to investigate the immune enhancement effects of the crude phenylethanoid glycosides (CPGs) from Ligustrum. Purpurascens on mice and analyze the chemical profiles of phenylethanoid glycosides in the CPGs.
The immune functions enhancing potential of CPGs was determined using serum hemolysin antibody, phagocytosis, splenocyte antibody production, and NK cells activity assays. The contents of five major constituents in the crude glycosides of Ligustrum purpurascens were determined by using liquid chromatography, other five glycosides were deduced according to their UV and MS spectra compared with the literature as well.
In the immunizing experiment, mice treated with different doses of CPGs showed an increase (p<0.01) in the haemagglutination titre compared with the control group. The increases (p<0.05) were found to be significant at doses of 440 mg/kg and 1.32 g/kg in the experiments of antibody production of spleen cells, MΦ phagocytosis of chicken RBCs and NK cell activity. Further chemical characterization yielded 10 constituents from CPGs, five glycosides were quantified by HPLC and the structures of other five compounds were speculated according to their UV and MS spectra.
The results suggested that phenylethanoid glycosides from Ligustrum purpurascens have immunomodulatory effects on mice.
女贞子，名为“苦丁茶”，在中国南方已被用作一种功能性茶约两千年，具有利尿、降血压、减肥和抗炎作用。
本研究旨在探讨女贞子中苯乙醇苷类（CPGs）对小鼠的免疫增强作用，并分析 CPGs 中苯乙醇苷的化学特征。
采用血清溶血素抗体、吞噬作用、脾细胞抗体产生和 NK 细胞活性测定法测定 CPGs 的免疫功能增强潜力。采用液相色谱法测定女贞子粗糖苷中五种主要成分的含量，根据紫外和质谱与文献比较推断出其他五种糖苷。
在免疫实验中，与对照组相比，不同剂量 CPGs 处理的小鼠的血凝滴度均升高（p<0.01）。在脾细胞抗体产生、MΦ 吞噬鸡 RBC 和 NK 细胞活性实验中，在 440mg/kg 和 1.32g/kg 剂量下，增加具有显著性（p<0.05）。进一步的化学特征分析从 CPGs 中得到 10 种成分，用 HPLC 定量了 5 种糖苷，根据紫外和质谱推测了其他 5 种化合物的结构。
结果表明，女贞子中的苯乙醇苷类对小鼠具有免疫调节作用。
