China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2013 Jun;63(Pt 6):2032-2037. doi: 10.1099/ijs.0.045534-0. Epub 2012 Oct 12.
A non-motile, Gram-stain-negative bacterium, designated R-HLS-17(T), was isolated from the meltwater of Hailuogou Glacier located in Sichuan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Flavobacterium, with the closest relatives being Flavobacterium antarcticum JCM 12383(T) (95.5% 16S rRNA gene sequence similarity), F. omnivorum JCM 11313(T) (95.0%) and F. fryxellicola LMG 22022(T) (95.2%). Growth occurred at 0-29 °C (optimum, 10-20 °C) and pH 6.0-8.5 (optimum, 7.0-8.0). The DNA G+C content was 46.5 mol%. The major cellular fatty acids were iso-C15:0, iso-C15:1 G, summed feature 9 (comprising iso-C17:1ω9c and/or 10-methyl C16:0), iso-C17:0 3-OH and iso-C15:0 3-OH. The predominant menaquinone was MK-6. Based on the genotypic and phenotypic characteristics, we propose that strain R-HLS-17(T) represents a novel species of the genus Flavobacterium, Flavobacterium noncentrifugens sp. nov. The type strain is R-HLS-17(T) (=CGMCC 1.10076(T)=NBRC 108844(T)).
一株非运动、革兰氏阴性细菌，命名为 R-HLS-17(T)，从位于中国西南部四川省的海螺沟冰川的融水中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，该分离株属于黄杆菌属，与最接近的亲缘种是南极黄杆菌 JCM 12383(T)（95.5% 16S rRNA 基因序列相似性）、F. omnivorum JCM 11313(T)（95.0%）和 F. fryxellicola LMG 22022(T)（95.2%）。在 0-29°C（最佳温度为 10-20°C）和 pH6.0-8.5（最佳 pH 值为 7.0-8.0）下生长。DNA G+C 含量为 46.5 mol%。主要细胞脂肪酸为 iso-C15:0、iso-C15:1 G、特征性脂肪酸 9（包括 iso-C17:1ω9c 和/或 10-甲基 C16:0）、iso-C17:0 3-OH 和 iso-C15:0 3-OH。主要的menaquinone 是 MK-6。基于基因型和表型特征，我们建议菌株 R-HLS-17(T)代表黄杆菌属的一个新种，Flavobacterium noncentrifugens sp. nov.。模式菌株是 R-HLS-17(T)=CGMCC 1.10076=NBRC 108844(T)。
