Zhang Yumin, Jiang Fan, Chang Xulu, Qiu Xia, Ren Lvzhi, Qu Zhihao, Deng Sangsang, Da Xuyang, Fang Chengxiang, Peng Fang
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Int J Syst Evol Microbiol. 2016 Jan;66(1):172-177. doi: 10.1099/ijsem.0.000688. Epub 2015 Oct 15.
A novel rod-shaped, Gram-stain-negative, non-gliding and aerobic strain surrounded by a multilayer capsule, designated 4-T-2T, was isolated from a till sample of Collins glacier front, Antarctica. The bacterium formed yellow, circular, convex and smooth colonies. Growth occurred at 4-28 °C (optimum18-20 °C), at pH 7.0-10.0 (optimum pH 9.0) and with 0-1 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 4-T-2T belonged to the genus Flavobacterium. Strain 4-T-2T shared the highest 16S rRNA gene sequence similarity with the type strain of Flavobacterium algicola (96.7 %). The DNA G+C content of strain 4-T-2T was 36.2 mol%. The only menaquinone was MK-6. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C16 : 0 and summed feature 9 (comprising iso-C17 : 1ω9c and/or 10-methyl C16 : 0). Polar lipid profile consisted phosphatidylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid and one unidentified lipid. On the basis of phylogenetic, physiological and chemotaxonomic data, strain 4-T-2T is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium collinsense sp. nov. is proposed. The type strain is 4-T-2T ( = CCTCC AB 2014004T = LMG 28257T).
从南极洲柯林斯冰川前沿的一个耕层样本中分离出了一株新型杆状、革兰氏染色阴性、不滑动且需氧的菌株，其周围有一层多层荚膜，命名为4-T-2T。该细菌形成黄色、圆形、凸起且光滑的菌落。在4-28°C（最适温度18-20°C）、pH 7.0-10.0（最适pH 9.0）以及0-1% NaCl条件下生长。基于16S rRNA基因序列的系统发育分析表明，菌株4-T-2T属于黄杆菌属。菌株4-T-2T与嗜藻黄杆菌模式菌株的16S rRNA基因序列相似度最高（96.7%）。菌株4-T-2T的DNA G+C含量为36.2 mol%。唯一的甲基萘醌是MK-6。主要的细胞脂肪酸为异-C15:0、异-C15:1 G、异-C16:0以及总和特征9（包括异-C17:1ω9c和/或10-甲基-C16:0）。极性脂质谱由磷脂酰乙醇胺、一种未鉴定的氨基脂质、一种未鉴定的氨基磷脂和一种未鉴定的脂质组成。基于系统发育、生理和化学分类学数据，菌株4-T-2T被认为代表黄杆菌属的一个新物种，为此提出了新名称嗜柯林斯黄杆菌（Flavobacterium collinsense sp. nov.）。模式菌株为4-T-2T（=CCTCC AB 2014004T=LMG 28257T）。
