China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2019 Dec;69(12):3812-3817. doi: 10.1099/ijsem.0.003687.
A Gram-stain-negative, aerobic, rod-shaped, devoid of flagella, orange-yellow-coloured bacterium (strain LB2P22) was isolated from an ice sample collected from the Laigu glacier on the Tibetan Plateau, PR China. The results of 16S rRNA gene sequence analysis indicated that strain LB2P22 belongs to the genus with highest similarity to LB2P30 (98.14 %), CGMCC 1.5380 (98.02 %), CCM 8827 (97.94 %) and CGMCC 1.10125 (97.91 %). Strain LB2P22 had 77.99-84.99 % average nucleotide identity and 22.3-29.3 % digital DNA-DNA hybridization values with its closest relatives, indicating that it represents a novel species of the genus . Strain LB2P22 grew at 0-25 °C, pH 7.0-8.0 and up to 1.0 % (w/v) NaCl. The main cellular fatty acids were iso-C, summed feature 3 (comprising Cω7/Cω6) and anteiso-C. The genomic DNA G+C content is 34.3 mol%. The predominant menaquinone was MK-6 and the polar lipids were phosphatidylethanolamine, one unidentified aminolipid and four unidentified lipids. Based on these data, a novel species, sp. nov., is proposed, with LB2P22 (=CGMCC 1.11361=NBRC 113777) as the type strain.
从中国青藏高原来古冰川采集的冰样本中分离到一株革兰氏阴性、需氧、杆状、无鞭毛、呈橙黄色的细菌（菌株 LB2P22）。16S rRNA 基因序列分析结果表明，菌株 LB2P22 属于 属，与 LB2P30（98.14%）、CGMCC 1.5380（98.02%）、CCM 8827（97.94%）和 CGMCC 1.10125（97.91%）的相似性最高。菌株 LB2P22 与最接近的亲缘菌株的平均核苷酸同一性为 77.99-84.99%，数字 DNA-DNA 杂交值为 22.3-29.3%，表明其代表了属的一个新种。菌株 LB2P22 在 0-25°C、pH7.0-8.0 和高达 1.0%（w/v）NaCl 的条件下生长。主要细胞脂肪酸为 iso-C、summed feature 3（包含 Cω7/Cω6）和 anteiso-C。基因组 DNA G+C 含量为 34.3mol%。主要的menaquinone 为 MK-6，极性脂包括磷脂酰乙醇胺、一种未鉴定的胺脂和四种未鉴定的脂类。基于这些数据，提出了一个新的种，即 sp. nov.，LB2P22（=CGMCC 1.11361=NBRC 113777）为其模式菌株。
