Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, New York, USA.
Nat Struct Mol Biol. 2012 Nov;19(11):1182-4. doi: 10.1038/nsmb.2405. Epub 2012 Oct 14.
Coenzyme B(12) has a key role in various enzymatic reactions and controls expression of bacterial genes through riboswitches. Here we report the crystal structure of the Symbiobacterium thermophilum B(12) riboswitch bound to its ligand adenosylcobalamin. The riboswitch forms a unique junctional structure with a large ligand-binding pocket tailored for specific recognition of the adenosyl moiety and flanked by structural elements that stabilize the regulatory region and enable control of gene expression.
辅酶 B(12) 在各种酶反应中起着关键作用，并通过核糖体开关控制细菌基因的表达。在这里，我们报告了与配体腺苷钴胺素结合的嗜热栖热菌 B(12) 核糖体开关的晶体结构。该核糖体开关形成了一个独特的连接结构，具有一个大型配体结合口袋，专门用于对腺苷部分进行特异性识别，并由稳定调节区域和控制基因表达的结构元件侧翼。
