Kurapati Kesava Rao V, Samikkannu Thangavel, Kadiyala Dakshayani B, Zainulabedin Saiyed M, Gandhi Nimisha, Sathaye Sadhana S, Indap Manohar A, Boukli Nawal, Rodriguez Jose W, Nair Madhavan P N
Department of Immunology, Florida International University, Miami, FL, USA.
J Basic Clin Physiol Pharmacol. 2012;23(4):139-46. doi: 10.1515/jbcpp-2012-0021.
Many plant-derived products exhibit potent chemopreventive activity against animal tumor models as well as rodent and human cancer cell lines. They have low side effects and toxicity and presumably modulate the factors that are critical for cell proliferation, differentiation, senescence and apoptosis. The present study investigates the effects of some medicinal plant extracts from generally recognized as safe plants that may be useful in the prevention and treatment of cancer.
Clonogenic assays using logarithmically-growing cells were performed to test the effect. The cytotoxic effects of Curcuma longa and Zingiber officinale were studied using sulforhodamine B assay, tetrazolium dye assay, colony morphology and microscopic analysis.
Out of the 13 lyophilized plant-derived extracts evaluated for growth-inhibitory effects on the PC-3M prostate cancer cell line, two extracts derived from C. longa and Z. officinale showed significant inhibitory effects on colony-forming ability. The individual and augmentative effects of these two extracts were tested for their narrow range effective lower concentration on PC-3M in clonogenic assays. At relatively lower concentrations, C. longa showed significant inhibition of colony formation in clonogenic assays; whereas at same concentrations Z. officinale showed only moderate inhibitory effects. However, when both the agents were tested together at the same concentrations, the combined effects were much more significant than their individual ones. On normal prostate epithelial cells both C. longa and Z. officinale had similar effects but at a lower magnitude. These observations were confirmed by several cytotoxicity assays involving the morphological appearance of the colonies, microscopic observations, per cent inhibition in comparison to control by sulforhodamine B and tetrazolium dye assay.
From these observations, it was concluded that the combined effects of C. longa and Z. officinale are much greater than their individual effects, suggesting the role of multiple components and their synergistic mode of actions to elicit stronger beneficial effects.
许多植物衍生产品对动物肿瘤模型以及啮齿动物和人类癌细胞系具有强大的化学预防活性。它们具有低副作用和低毒性，并且可能调节对细胞增殖、分化、衰老和凋亡至关重要的因素。本研究调查了一些来自普遍认为安全的植物的药用植物提取物在癌症预防和治疗中的作用。
使用对数生长期细胞进行克隆形成试验以测试效果。使用磺基罗丹明B试验、四唑盐染料试验、集落形态和显微镜分析研究了姜黄和生姜的细胞毒性作用。
在评估对PC-3M前列腺癌细胞系生长抑制作用的13种冻干植物衍生提取物中，两种来自姜黄和生姜的提取物对集落形成能力显示出显著的抑制作用。在克隆形成试验中，测试了这两种提取物在窄范围有效较低浓度下对PC-3M的单独和增强作用。在相对较低浓度下，姜黄在克隆形成试验中显示出对集落形成的显著抑制；而在相同浓度下，生姜仅显示出中等抑制作用。然而，当两种药物在相同浓度下一起测试时，联合作用比它们各自的作用更显著。对正常前列腺上皮细胞，姜黄和生姜具有相似的作用，但程度较低。这些观察结果通过几种细胞毒性试验得到证实，包括集落的形态外观、显微镜观察、与磺基罗丹明B和四唑盐染料试验对照相比的抑制百分比。
从这些观察结果得出结论，姜黄和生姜的联合作用远大于它们各自的作用，表明多种成分的作用及其协同作用模式可产生更强的有益效果。
