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迷迭香（唇形科）、姜黄（姜科）和生姜（姜科）精油对子宫颈癌细胞（HeLa）细胞毒性活性的评估

Assessment of Cytotoxic Activity of Rosemary ( L.), Turmeric ( L.), and Ginger ( R.) Essential Oils in Cervical Cancer Cells (HeLa).

作者信息

Santos P A S R, Avanço G B, Nerilo S B, Marcelino R I A, Janeiro V, Valadares M C, Machinski Miguel

机构信息

Laboratory of Toxicology, Department of Basic Health Sciences, State University of Maringá, Maringá, PR, Brazil.

University Center Inga (UNINGÁ), Maringá, PR, Brazil.

出版信息

ScientificWorldJournal. 2016;2016:9273078. doi: 10.1155/2016/9273078. Epub 2016 Nov 30.

DOI:10.1155/2016/9273078
PMID:28042599
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5155122/
Abstract

The objective of this study was to evaluate the cytotoxic activity of rosemary (REO, L.), turmeric (CEO, L.), and ginger (GEO, R.) essential oils in HeLa cells. Cytotoxicity tests were performed , using tetrazolium (MTT) and neutral red assays for evaluation of antiproliferative activity by different mechanisms, trypan blue assay to assess cell viability and evaluation of cell morphology for Giemsa to observe the cell damage, and Annexin V to evaluate cell death by apoptosis. CEO and GEO exhibited potent cytotoxic activity against HeLa cells. IC obtained was 36.6 g/mL for CEO and 129.9 g/mL for GEO. The morphology of HeLa cells showed condensation of chromatin, loss of cell membrane integrity with protrusions (blebs), and cell content leakage for cells treated with CEO and GEO, from the lowest concentrations studied, 32.81 g/mL of CEO and 32.12 g/mL of GEO. The Annexin V assay revealed a profile of cell death by apoptosis for both CEO and GEO. The results indicate cytotoxic activity for CEO and GEO, suggesting potential use as anticancer agents for cervical cancer cells.

摘要

本研究的目的是评估迷迭香（REO，唇形科）、姜黄（CEO，姜科）和生姜（GEO，姜科）精油对人宫颈癌HeLa细胞的细胞毒性活性。进行了细胞毒性试验，使用四氮唑（MTT）和中性红试验通过不同机制评估抗增殖活性，用台盼蓝试验评估细胞活力并通过吉姆萨染色评估细胞形态以观察细胞损伤，还用膜联蛋白V评估细胞凋亡导致的细胞死亡。CEO和GEO对HeLa细胞表现出强大的细胞毒性活性。CEO的半数抑制浓度（IC）为36.6μg/mL，GEO的为129.9μg/mL。HeLa细胞的形态显示，从所研究的最低浓度（CEO为32.81μg/mL，GEO为32.12μg/mL）开始，用CEO和GEO处理的细胞出现染色质凝聚、细胞膜完整性丧失并伴有突起（泡状）以及细胞内容物泄漏。膜联蛋白V试验显示CEO和GEO均呈现细胞凋亡导致的细胞死亡特征。结果表明CEO和GEO具有细胞毒性活性，提示其有可能用作子宫颈癌细胞的抗癌剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8324/5155122/74f5345258fc/TSWJ2016-9273078.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8324/5155122/0410cd191e51/TSWJ2016-9273078.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8324/5155122/5d854c9e1a4f/TSWJ2016-9273078.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8324/5155122/74f5345258fc/TSWJ2016-9273078.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8324/5155122/0410cd191e51/TSWJ2016-9273078.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8324/5155122/5d854c9e1a4f/TSWJ2016-9273078.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8324/5155122/74f5345258fc/TSWJ2016-9273078.003.jpg

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