Simonet M, Richard S, Berche P
Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, Paris, France.
Infect Immun. 1990 Mar;58(3):841-5. doi: 10.1128/iai.58.3.841-845.1990.
Electron microscopic evidence is presented that bacteria harboring the virulence plasmid pYV from Yersinia pseudotuberculosis are localized in extracellular sites during the course of infection in mice, often unambiguously undergoing active replication. Virulent pYV+ bacteria, often seen adherent to platelets, severely restricted granuloma formation, creating necrotic microabscesses poorly populated with inflammatory cells. This contrasts with granulomas produced by pYV- bacteria, which appear to be composed mainly of polymorphonuclear and mononuclear cells. Our results therefore strongly suggest that active replication of pYV+ bacteria predominantly, if not exclusively, occurs in vivo in extracellular sites.
有电子显微镜证据表明，携带来自假结核耶尔森氏菌的毒力质粒pYV的细菌在小鼠感染过程中定位于细胞外部位，常常明显地进行活跃复制。有毒力的pYV+细菌常常可见附着于血小板，严重限制肉芽肿形成，产生坏死性微脓肿，其中炎性细胞数量很少。这与pYV-细菌产生的肉芽肿形成对比，后者似乎主要由多形核细胞和单核细胞组成。因此，我们的结果强烈表明，pYV+细菌的活跃复制主要（如果不是唯一的话）在体内的细胞外部位发生。
