Laboratorio de Fisiología (IHEM-CONICET), and Departamento de Morfología y Fisiología (Facultad de Ciencias Médicas, Universidad Nacional de Cuyo), Casilla de Correo 33, 5500 Mendoza, Argentina.
J Exp Biol. 2013 Feb 15;216(Pt 4):614-22. doi: 10.1242/jeb.075655. Epub 2012 Oct 17.
The invasive Pomacea canaliculata estivates during periods of drought and should cope with harmful effects of reoxygenation during arousal. We studied thiobarbituric acid reactive substances (TBARS), enzymatic (superoxide dismutase, SOD and catalase, CAT) and non-enzymatic antioxidants (uric acid and reduced glutathione), and heat shock protein expression (Hsc70, Hsp70 and Hsp90) in (1) active control snails, (2) snails after 45 days of estivation, and (3) aroused snails 20 min and (4) 24 h after water exposure, in midgut gland, kidney and foot. Both kidney and foot (but not the midgut gland) showed a TBARS increase during estivation and a decrease after arousal. Tissue SOD and CAT did not change in any experimental groups. Uric acid increased during estivation in all tissues, and it decreased after arousal in the kidney. Allantoin, the oxidation product of uric acid, remained constant in the midgut gland but it decreased in the kidney until 20 min after arousal; however, allantoin levels rose in both kidney and foot 24 h after arousal. Reduced glutathione decreased during estivation and arousal, in both midgut gland and kidney, and it remained constant in the foot. Hsc70 and Hsp70 kidney levels were stable during the activity-estivation cycle and Hsp90 expression decreases during estivation and recovers in the early arousal. In foot, the expression of Hsp70 and Hsp90 was high during activity and estivation periods and disminished after arousal. Results indicate that a panoply of antioxidant and molecular chaperone defences may be involved during the activity-estivation cycle in this freshwater gastropod.
入侵性Pomacea canaliculata 在干旱期间会夏眠，并应在苏醒时应对再氧化的有害影响。我们研究了硫代巴比妥酸反应物质 (TBARS)、酶 (超氧化物歧化酶、SOD 和过氧化氢酶、CAT) 和非酶抗氧化剂 (尿酸和还原型谷胱甘肽) 以及热休克蛋白表达 (Hsc70、Hsp70 和 Hsp90) 在 (1) 活跃对照组蜗牛、(2) 夏眠 45 天后的蜗牛和 (3) 苏醒后 20 分钟和 (4) 水暴露后 24 小时的中肠腺、肾脏和足部。肾脏和足部（但不是中肠腺）在夏眠期间 TBARS 增加，苏醒后减少。组织 SOD 和 CAT 在任何实验组中都没有变化。尿酸在夏眠期间在所有组织中增加，在苏醒后在肾脏中减少。尿酸的氧化产物尿囊素在中肠腺中保持不变，但在肾脏中在苏醒后 20 分钟下降；然而，尿囊素水平在苏醒后 24 小时在肾脏和足部上升。还原型谷胱甘肽在夏眠和苏醒期间减少，在中肠腺和肾脏中保持不变，在足部保持不变。Hsc70 和 Hsp70 肾脏水平在活动-休眠周期中保持稳定，Hsp90 表达在休眠期间下降并在早期苏醒时恢复。在足部，Hsp70 和 Hsp90 的表达在活动和休眠期间较高，苏醒后下降。结果表明，在这种淡水腹足类动物的活动-休眠周期中，可能涉及多种抗氧化和分子伴侣防御机制。
