Fang Miao, Yu Fandong, Shu Lu, Wei Hui, Mu Xidong, Wang Xuejie, Xu Meng, Gu Dangen
Pearl River Fisheries Research Institute Chinese Academy of Fishery Sciences Guangzhou China.
Key Laboratory of Prevention and Control for Aquatic Invasive Alien Species Ministry of Agriculture and Rural Affairs Guangzhou China.
Ecol Evol. 2024 Oct 3;14(10):e70283. doi: 10.1002/ece3.70283. eCollection 2024 Oct.
Gut microbiota is related to host fitness, and influenced by geographical locations and habitats. is a malignant invasive alien snail that threatens agricultural production and ecosystem functions worldwide. Clarifying the general rules of the gut microbial community structure and function of the snails in different geographical locations and habitats is of great significance for understanding their invasion at different spatial scales. This study used high-throughput sequencing technology to compare and analyze the differences in community structure and function of gut microbiota in from five geographical locations (Liuzhou, Yulin, Nanning, Wuzhou, and Hezhou) and three different habitats (pond, paddy field, and ditch) in Guangxi Province. The results showed that intestinal microbial alpha diversity of was higher in Liuzhou, Yulin, lower in Nanning, Wuzhou, Hezhou, and higher in ponds compared with paddy fields and ditches. The dominant phyla of gut microbiota in snails were Firmicutes, Cyanobacteria, Proteobacteria, Fusobacteriota, Bacteroidota, and the dominant genus was . The community structure of gut microbiota in snails varied significantly across different geographical locations and habitats, and the phyla Firmicutes, Cyanobacteria had significantly higher relative abundance in snails collected from Nanning and Yulin, respectively. Moreover, the relative abundance of gut functional microbiota associated with human disease in was significantly affected by geographical locations and habitats, and with the highest abundance in ponds. However, the relative abundance of functional microbiota related to metabolism, genetic information processing, organizational system, environmental information processing, and cellular processes were only significantly affected by geographical locations. Collectively, geographical locations and habitats had significantly different effects on the community structure and function of gut microbiota in , and the greater differences were caused by geographical locations rather than by habitats.
肠道微生物群与宿主健康相关，并受地理位置和栖息地的影响。福寿螺是一种恶性入侵外来螺类，威胁着全球农业生产和生态系统功能。阐明不同地理位置和栖息地的福寿螺肠道微生物群落结构和功能的一般规律，对于理解其在不同空间尺度上的入侵具有重要意义。本研究利用高通量测序技术，对广西五个地理位置（柳州、玉林、南宁、梧州和贺州）和三种不同栖息地（池塘、稻田和沟渠）的福寿螺肠道微生物群的群落结构和功能差异进行了比较分析。结果表明，柳州、玉林的福寿螺肠道微生物α多样性较高，南宁、梧州、贺州的较低，与稻田和沟渠相比，池塘中的福寿螺肠道微生物α多样性更高。福寿螺肠道微生物群的优势菌门为厚壁菌门、蓝细菌门、变形菌门、梭杆菌门、拟杆菌门，优势菌属为 。福寿螺肠道微生物群的群落结构在不同地理位置和栖息地之间存在显著差异，厚壁菌门、蓝细菌门在分别从南宁和玉林采集的福寿螺中相对丰度显著较高。此外，福寿螺中与人类疾病相关的肠道功能微生物群的相对丰度受地理位置和栖息地的显著影响，在池塘中丰度最高。然而，与代谢、遗传信息处理、组织系统、环境信息处理和细胞过程相关的功能微生物群的相对丰度仅受地理位置的显著影响。总体而言，地理位置和栖息地对福寿螺肠道微生物群的群落结构和功能有显著不同的影响，且差异较大是由地理位置而非栖息地造成的。
