Korsuwannawong Suwanna, Srichan Ratchaporn, Vajrabhaya La-Ongthong
Research Service Center, Faculty of Dentistry, Mahidol University, Yothi Street, Rachatavee, Bangkok, 10400 THAILAND.
Eur J Dent. 2012 Oct;6(4):408-14.
The aim of this study was to evaluate the cytotoxicity of three dentine bonding agents (G-Bond, Clearfil S(3) Bond and Clearfil SE Bond X) in cell-culture perfusion.
In this experiment, 8×10(4) TCPC SV40 cells (bovine-pulp-derived cells transfected with simian virus 40 large T-antigen) in MEM-alpha media, 20%FCS were seeded on mesh in a 6-well plate and incubated at 37 °C with 5% CO(2). After 2 days, the mesh inserts were transferred to a 24-well plate and incubated in MEM-alpha media, 20%FCS with 50 μg/ml of ascorbic acid at 37 °C with 5% CO(2) for 14 days. The test materials were applied to the dentine discs of dentine barrier models. Three-dimensional cell cultures in mesh and perfusion conditions were generated in this experiment. Each material, as well as the negative control (President) and the positive control (vitrebond), was tested in 5 models with the tests repeated in triplicate. The MTT assay was used to determine cell viability after the diffusion of leachable toxicity from the tested materials through the dentine discs.
The cell survival rate with G-Bond and Clearfil S(3) Bond was 113.03 and 90.98 percent, respectively, whereas that with Clearfil SE Bond X was 111.83 percent. All three dentine bonding agents had no toxicity compared with the negative control group (P>.05).
All three self-etching dentine-bonding agents are nontoxic. Pulp damage caused by these three bonding agents is unlikely to occur in the clinic.
本研究旨在评估三种牙本质粘结剂（G-Bond、Clearfil S(3) Bond和Clearfil SE Bond X）在细胞培养灌注中的细胞毒性。
在本实验中，将8×10⁴个处于含20%胎牛血清（FCS）的α-改良 Eagle培养基（MEM-α）中的TCPC SV40细胞（转染了猿猴病毒40大T抗原的牛牙髓来源细胞）接种于6孔板中的网片上，并在37℃、5%二氧化碳条件下孵育。2天后，将网片移至24孔板中，在含20%FCS、50μg/ml抗坏血酸的MEM-α培养基中于37℃、5%二氧化碳条件下孵育14天。将测试材料应用于牙本质屏障模型的牙本质盘上。本实验在网片和灌注条件下进行三维细胞培养。每种材料以及阴性对照（President）和阳性对照（vitrebond）均在5个模型中进行测试，测试重复三次。通过牙本质盘扩散测试材料的可浸出毒性后，采用MTT法测定细胞活力。
G-Bond和Clearfil S(3) Bond的细胞存活率分别为113.03%和90.98%，而Clearfil SE Bond X的细胞存活率为111.83%。与阴性对照组相比，所有三种牙本质粘结剂均无毒性（P>0.05）。
所有三种自酸蚀牙本质粘结剂均无毒。在临床上，这三种粘结剂不太可能导致牙髓损伤。
