Schmalz G, Garhammer P, Schweiki H
Clinic of Operative Dentistry and Periodontology, University of Regensburg, Germany.
J Endod. 1996 May;22(5):249-52. doi: 10.1016/s0099-2399(06)80142-1.
The suitability of a dentin barrier test based on a commercially available cell culture chamber was evaluated by testing the cytotoxicity of dental cements. The two chambers of the culture device as produced are separated by a membrane. This was replaced by a bovine dentin disk (500 micrometers thick). Mouse fibroblasts were grown on the "pulpal" side of the dentin for 24 h; test materials were then placed into the "cavity" side of the upper chamber. The number of viable cells was determined after 24 h. After exposure to zinc phosphate cement at a powder/liquid ratio of 2:1, approximately 100% of cells survived. A ratio of 1:1 yielded 81% survival. Only 24% and 28% of the cells survived after exposure to Ketac Fil and Ketac Silver, respectively. The light-curing glass ionomer cement (vitrebond) and zinc oxide-eugenol killed all cells. These results agree with those obtained from a previous study, wherein the dentin barrier test device was constructed in our laboratory.
通过测试牙科粘固剂的细胞毒性，评估了基于市售细胞培养室的牙本质屏障测试的适用性。所生产的培养装置的两个腔室由一个膜隔开。用牛牙本质盘（500微米厚）代替了该膜。将小鼠成纤维细胞在牙本质的“牙髓”侧培养24小时；然后将测试材料放入上腔室的“洞腔”侧。24小时后测定活细胞数量。以2:1的粉液比接触磷酸锌粘固剂后，约100%的细胞存活。1:1的比例产生81%的存活率。接触Ketac Fil和Ketac Silver后，分别只有24%和28%的细胞存活。光固化玻璃离子粘固剂（vitrebond）和氧化锌丁香酚杀死了所有细胞。这些结果与我们实验室构建牙本质屏障测试装置的先前研究结果一致。
