Schmalz G, Schuster U, Nuetzel K, Schweikl H
Department of Operative Dentistry and Periodontology, University of Regensburg, Germany.
J Endod. 1999 Jan;25(1):24-9. doi: 10.1016/S0099-2399(99)80394-X.
To better simulate the in vivo situation, a three-dimensional fibroblast cell culture was introduced into an in vitro pulp chamber model. The system was evaluated by testing a series of dental filling materials. After a 24-h exposure with (0.3 or 5 ml/h) and without perfusion of the pulp chamber, the tissues were subjected to a routine MTT assay. Zinc phosphate cement, conventional glass ionomer cements, a silicone impression material, and zinc oxide-eugenol did not influence cell viability, compared with untreated controls; but, a light-curing glass ionomer cement significantly reduced cell survival. Perfusion of the chambers did not significantly influence the results, but perfusion conditions of 5 ml/h lead to a general decrease of cell vitality. The three-dimensional cell culture system in an in vitro pulp chamber seems to be a substantial improvement, because zinc oxide-eugenol does not evoke a cellular reaction (as is the case in vivo), and the test system is sensitive enough to detect other toxicants.
为了更好地模拟体内情况，将三维成纤维细胞培养引入体外牙髓腔模型。通过测试一系列牙科填充材料对该系统进行评估。在牙髓腔灌注（0.3或5 ml/h）和不灌注的情况下暴露24小时后，对组织进行常规MTT测定。与未处理的对照组相比，磷酸锌水门汀、传统玻璃离子水门汀、硅橡胶印模材料和氧化锌丁香酚对细胞活力没有影响；但是，光固化玻璃离子水门汀显著降低了细胞存活率。牙髓腔的灌注对结果没有显著影响，但5 ml/h的灌注条件导致细胞活力普遍下降。体外牙髓腔中的三维细胞培养系统似乎有了实质性的改进，因为氧化锌丁香酚不会引发细胞反应（体内情况也是如此），并且该测试系统足够灵敏以检测其他有毒物质。
