Department of Medical Physics, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran ; Faculty of Science, University of Geneve, Geneve, Switzerland.
Radiol Oncol. 2012 Sep;46(3):226-32. doi: 10.2478/v10019-012-0035-x. Epub 2012 Jun 19.
The aim of this study was to investigate the antitumor effectiveness of electrochemotherapy with cisplatin combined with suboptimal radiotherapy doses. Tumor radiosensitization was evaluated on large invasive ductal carcinoma tumors in Balb/C mice.
Tumors of an average volume of 630 mm(3) were treated with cisplatin, electric pulses, radiotherapy, electrochemotherapy, alone as well as in appropriate combinations. Tumors were irradiated with Cobalt-60 γ-rays at doses 3 Gy and 5 Gy in combination with electrochemotherapy using cisplatin. Controls included each of the treatments alone as well as the combination of the radiotherapy with electric pulses alone or with cisplatin alone. Antitumor effectiveness was evaluated by tumor growth delay, tumor-doubling time, inhibition ratio and the objective response rates.
As anticipated, electrochemotherapy was more effective than the treatment with cisplatin alone or the application of the electric pulses alone. When treatments were combined with tumor irradiation at either 3 or 5 Gy, the combination with electrochemotherapy was more effective: at 5 Gy, 2 animals out of 8 were in complete remission 100 days later. In general the higher 5 Gy dose of γ-radiation was more effective than the lower one of 3 Gy.
The results of our study demonstrate that irradiation doses, 3 Gy or 5 Gy, increase the antitumor effectiveness of electrochemotherapy with cisplatin on invasive ductal carcinoma tumors. Good antitumor results were achieved in experimental tumors with a size comparable to clinical lesions, demonstrating that this three-modality combined treatment is useful for the treatment of large lesions even at sub-optimal radiotherapy doses.
本研究旨在探讨顺铂电化学疗法联合亚致死剂量放疗的抗肿瘤疗效。在 Balb/C 小鼠的大型浸润性导管癌肿瘤中评估肿瘤放射增敏作用。
平均体积为 630mm³的肿瘤分别用顺铂、电脉冲、放疗、电化学疗法单独以及适当组合进行治疗。肿瘤用钴-60γ射线照射，剂量为 3Gy 和 5Gy，联合顺铂电化学疗法。对照组包括每种治疗方法单独以及放疗与电脉冲单独或与顺铂联合的组合。通过肿瘤生长延迟、肿瘤倍增时间、抑制率和客观缓解率评估抗肿瘤疗效。
正如预期的那样，电化学疗法比单独使用顺铂或单独使用电脉冲更有效。当与 3 或 5Gy 的肿瘤照射联合治疗时，与电化学疗法联合治疗更有效：在 5Gy 时，8 只动物中有 2 只在 100 天后完全缓解。一般来说，较高的 5Gyγ射线剂量比较低的 3Gy 更有效。
我们的研究结果表明，照射剂量 3Gy 或 5Gy 增加了顺铂电化学疗法对浸润性导管癌肿瘤的抗肿瘤疗效。在与临床病变大小相当的实验性肿瘤中取得了良好的抗肿瘤效果，表明这种三模态联合治疗对于治疗大病变即使在亚致死剂量的放疗下也是有用的。
