Cemazar M, Milacic R, Miklavcic D, Dolzan V, Sersa G
Department of Tumor Biology, Institute of Oncology, Ljubljana, Slovenia.
Anticancer Drugs. 1998 Jul;9(6):525-30. doi: 10.1097/00001813-199807000-00002.
Electrochemotherapy using intratumoral cisplatin administration was tested on EAT tumors in mice. Mice were treated with eight electric pulses (100 micros, 1 Hz, 1040 V) and/or cisplatin (1, 2, 4 and 8 mg/kg). Cisplatin treatment resulted in up to 20 days of tumor growth delay. Electrochemotherapy resulted in tumor cures; local tumor control reached a plateau at 4 mg/kg in 67% of tumor cures. The maximal effect of electrochemotherapy was achieved when cisplatin was injected 5 min before or simultaneously with electric pulses application. Approximately two times more platinum was bound to DNA in electrochemotherapy than in cisplatin treated tumors at all time points tested. Our study shows that electrochemotherapy with intratumoral cisplatin administration is a very effective local treatment of EAT tumors with high curability rate.
对小鼠的艾氏腹水瘤（EAT）进行了瘤内注射顺铂的电化学疗法试验。小鼠接受了8个电脉冲（100微秒，1赫兹，1040伏）和/或顺铂（1、2、4和8毫克/千克）治疗。顺铂治疗使肿瘤生长延迟长达20天。电化学疗法实现了肿瘤治愈；在67%的肿瘤治愈案例中，局部肿瘤控制在4毫克/千克时达到平台期。当在施加电脉冲前5分钟或同时注射顺铂时，可实现电化学疗法的最大效果。在所有测试时间点，电化学疗法中与DNA结合的铂比顺铂治疗的肿瘤中大约多两倍。我们的研究表明，瘤内注射顺铂的电化学疗法是一种治疗EAT肿瘤的非常有效的局部疗法，治愈率很高。
