Crumpacker C S, Schnipper L E, Zaia J A, Levin M J
Antimicrob Agents Chemother. 1979 May;15(5):642-5. doi: 10.1128/AAC.15.5.642.
Inhibition by acycloguanosine (ACG) of plaque formation by harpes simplex virus types 1 and 2 (HSV-1 and HSV-2), varicella-zoster virus, and cytomegalovirus was studied. Seventeen clinical isolates of HSV-1 were inhibited by ACG at a mean 50% inhibitory dose (ID(50)) of 0.15 +/- 0.09 muM. The mean ID(50) for 10 isolates of HSV-2 was 1.62 +/- 0.76 muM, and for four isolates of varicella-zoster virus it was 3.75 +/- 1.30 muM. The ID(50)'s for two cytomegalovirus isolates were 100 and 160 muM, and for four additional isolates of cytomegalovirus no end point (ID(50)) was reached at 200 muM. ACG at a concentration of 200 muM had no effect on deoxyribonucleic acid synthesis in human fibroblast cells and only inhibited thymidine incorporation by Vero cells by one-third. These studies demonstrated the antiviral activity of ACG against clinical isolates of HSV-1, HSV-2, and varicella-zoster virus and the lack of toxicity to monkey or human cells in culture at concentrations which markedly inhibited these viruses. ACG had little effect on cytomegalovirus at concentrations in excess of 100 muM.
研究了无环鸟苷（ACG）对1型和2型单纯疱疹病毒（HSV - 1和HSV - 2）、水痘 - 带状疱疹病毒及巨细胞病毒空斑形成的抑制作用。17株HSV - 1临床分离株被ACG抑制，平均50%抑制剂量（ID(50)）为0.15±0.09μM。10株HSV - 2分离株的平均ID(50)为1.62±0.76μM，4株水痘 - 带状疱疹病毒分离株的平均ID(50)为3.75±1.30μM。2株巨细胞病毒分离株的ID(50)分别为100和160μM，另外4株巨细胞病毒分离株在200μM时未达到终点（ID(50)）。浓度为200μM的ACG对人成纤维细胞中的脱氧核糖核酸合成无影响，仅使Vero细胞的胸苷掺入量抑制了三分之一。这些研究证明了ACG对HSV - 1、HSV - 2和水痘 - 带状疱疹病毒临床分离株具有抗病毒活性，且在明显抑制这些病毒的浓度下对培养的猴或人细胞无毒性。ACG在浓度超过100μM时对巨细胞病毒几乎没有作用。
