哺乳动物糖原在溶液中的¹H核磁共振可见性。
1H NMR visibility of mammalian glycogen in solution.
作者信息
Zang L H, Rothman D L, Shulman R G
机构信息
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06510.
出版信息
Proc Natl Acad Sci U S A. 1990 Mar;87(5):1678-80. doi: 10.1073/pnas.87.5.1678.
Abstract
High-resolution 1H NMR spectra of rabbit liver glycogen in 2H2O were obtained at 500 MHz, and several resonances were assigned by comparison with the chemical shifts of alpha-linked diglucose molecules. The NMR relaxation times T1 and T2 of glycogen in 2H2O were determined to be 1.1 and 0.029 s, respectively. The measured natural linewidth of the carbon-1 proton (12 +/- 2 Hz) is in excellent agreement with that calculated from T2. The visibility measurements made by digesting glycogen and comparing glucose and glycogen signal intensities demonstrate that in spite of the very high molecular weight, all of the proton nuclei in glycogen contribute to the NMR spectrum. The result is not unexpected, since 100% NMR visibility was previously observed from the carbon nuclei of glycogen, due to the rapid intramolecular motions.
摘要
在500兆赫下获得了兔肝糖原在重水中的高分辨率1H核磁共振谱，并通过与α-连接的二葡萄糖分子的化学位移进行比较，确定了几个共振峰。重水中糖原的核磁共振弛豫时间T1和T2分别测定为1.1秒和0.029秒。测得的碳-1质子的天然线宽（12±2赫兹）与根据T2计算得到的值非常吻合。通过消化糖原并比较葡萄糖和糖原信号强度进行的可见性测量表明，尽管糖原分子量非常高，但糖原中的所有质子核都对核磁共振谱有贡献。这一结果并不意外，因为由于分子内的快速运动，之前已观察到糖原的碳核具有100%的核磁共振可见性。
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本文引用的文献
1
Structure of maltoheptaose by difference Fourier methods and a model for glycogen.通过差值傅里叶方法解析麦芽七糖的结构及糖原模型
J Mol Biol. 1982 Apr 5;156(2):411-27. doi: 10.1016/0022-2836(82)90336-9.
2
Structure and metabolism of mammalian liver glycogen monitored by carbon-13 nuclear magnetic resonance.通过碳-13核磁共振监测哺乳动物肝脏糖原的结构与代谢。
Biochemistry. 1983 Mar 1;22(5):1087-94. doi: 10.1021/bi00274a015.
3
Rabbit skeletal muscle glycogen. A morphological and biochemical study of glycogen beta-particles isolated by the precipitation-centrifugation method.兔骨骼肌糖原。对通过沉淀离心法分离得到的糖原β颗粒的形态学和生物化学研究。
J Cell Biol. 1968 Jul;38(1):130-50. doi: 10.1083/jcb.38.1.130.
4
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5
Detection of human muscle glycogen by natural abundance 13C NMR.利用自然丰度13C核磁共振技术检测人体肌肉糖原。
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1634-6. doi: 10.1073/pnas.85.5.1634.
6
NMR measurements of in vivo myocardial glycogen metabolism.体内心肌糖原代谢的核磁共振测量
J Biol Chem. 1988 Feb 15;263(5):2285-91.
7
Natural-abundance 13C NMR study of glycogen repletion in human liver and muscle.人体肝脏和肌肉中糖原补充的天然丰度13C核磁共振研究
Proc Natl Acad Sci U S A. 1989 Mar;86(5):1439-42. doi: 10.1073/pnas.86.5.1439.
8
Hepatic glycogen synthesis from duodenal glucose and alanine. An in situ 13C NMR study.十二指肠葡萄糖和丙氨酸合成肝糖原。一项原位¹³C核磁共振研究。
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