利用自然丰度13C核磁共振技术检测人体肌肉糖原。
Detection of human muscle glycogen by natural abundance 13C NMR.
作者信息
Avison M J, Rothman D L, Nadel E, Shulman R G
机构信息
Department of Molecular Biophysics, Yale University School of Medicine, New Haven, CT 06520.
出版信息
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1634-6. doi: 10.1073/pnas.85.5.1634.
Abstract
Natural abundance 13C nuclear magnetic resonance spectroscopy was used to detect signals from glycogen in the human gastrocnemius muscle. The reproducibility of the measurement was demonstrated, and the ability to detect dynamic changes was confirmed by measuring a decrease in muscle glycogen levels after exercise and its subsequent repletion. Single frequency gated 1H decoupling was used to obtain decoupled natural abundance 13C NMR spectra of the C-1 position of muscle glycogen.
摘要
利用天然丰度13C核磁共振波谱法检测人腓肠肌中糖原的信号。证明了测量的可重复性，并通过测量运动后肌肉糖原水平的降低及其随后的补充，证实了检测动态变化的能力。使用单频门控1H去耦来获得肌肉糖原C-1位置的去耦天然丰度13C NMR谱。
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