Centre for Advanced Discovery and Experimental Therapeutics, Institute of Human Development, University of Manchester and Manchester Royal Eye Hospital, Central Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Science Centre, Oxford Road, Manchester, M13 9PT, UK.
Metallomics. 2012 Dec;4(12):1245-54. doi: 10.1039/c2mt20157g.
Inductively coupled plasma mass spectrometry (ICP-MS) was used to quantify the total amount of trace elements in retina from adult male Sprague-Dawley rats (n = 6). Concentration of trace elements within individual retinal areas in frozen sections of the fellow eye was established with the use of two methodologies: (1) particle-induced X-ray emission (PIXE) in combination with 3D depth profiling with Rutherford backscattering spectrometry (RBS) and (2) synchrotron X-ray fluorescence (SXRF) microscopy. The most abundant metal in the retina was zinc, followed by iron and copper. Nickel, manganese, chromium, cobalt, selenium and cadmium were present in very small amounts. The PIXE and SXRF analysis yielded a non-homogenous pattern distribution of metals in the retina. Relatively high levels of zinc were found in the inner part of the photoreceptor inner segments (RIS)/outer limiting membrane (OLM), inner nuclear layer and plexiform layers. Iron was found to accumulate in the retinal pigment epithelium/choroid layer and RIS/OLM. Copper in turn, was localised primarily in the RIS/OLM and plexiform layers. The trace elements iron, copper, and zinc exist in different amounts and locations in the rat retina.
电感耦合等离子体质谱法（ICP-MS）用于定量成年雄性 Sprague-Dawley 大鼠（n = 6）视网膜中的痕量元素总量。使用两种方法在同眼冷冻切片中确定单个视网膜区域内痕量元素的浓度：（1）粒子诱导 X 射线发射（PIXE）与卢瑟福背散射光谱法（RBS）的 3D 深度剖析相结合，（2）同步加速器 X 射线荧光（SXRF）显微镜。视网膜中最丰富的金属是锌，其次是铁和铜。镍、锰、铬、钴、硒和镉的含量非常少。PIXE 和 SXRF 分析显示出视网膜中金属的非均匀图案分布。相对较高水平的锌存在于光感受器内节（RIS）/外节限膜（OLM）、内核层和神经丛层的内部。铁被发现积聚在视网膜色素上皮/脉络膜层和 RIS/OLM 中。反过来，铜主要定位于 RIS/OLM 和神经丛层。在大鼠视网膜中，痕量元素铁、铜和锌的含量和位置不同。
