Hirayama Y
Department of Ophthalmology, Nagasaki University School of Medicine, Japan.
Acta Histochem. 1990;89(1):107-11. doi: 10.1016/S0065-1281(11)80328-4.
Zinc and copper were histochemically localized in rat ocular tissues. Zinc was demonstrated by the dithizone method, and copper by the rubeanic acid and rhodanine methods. The retinal photoreceptor's outer segment showed the presence of zinc, but no zinc reaction was seen in other parts of the retina or other ocular tissues, even in rats fed excess zinc. It seems that photoreceptor cells contain the highest concentration of zinc. Copper was seen in the corneal epithelium and endothelium, iris, ciliary body, lens epithelium, retinal outer nuclear layer, photoreceptor inner and outer segments, retinal pigment epithelium, choroid, sclera, and optic nerve. These trace elements seems to play roles as components of some metalloenzymes and may have other functions till now unknown.
采用组织化学方法对大鼠眼组织中的锌和铜进行了定位。用双硫腙法显示锌，用黑尿酸和罗丹宁法显示铜。视网膜光感受器的外段显示有锌存在，但即使在喂食过量锌的大鼠中，视网膜的其他部位或其他眼组织也未观察到锌反应。似乎光感受器细胞中锌的浓度最高。在角膜上皮和内皮、虹膜、睫状体、晶状体上皮、视网膜外核层、光感受器内段和外段、视网膜色素上皮、脉络膜、巩膜和视神经中可见铜。这些微量元素似乎作为某些金属酶的成分发挥作用，并且可能具有目前未知的其他功能。
