使用微珠对牙本质涎蛋白（DSP）进行定量评估——一种潜在的牙根吸收早期标志物。
Quantitive evaluation of dentin sialoprotein (DSP) using microbeads - a potential early marker of root resorption.
作者信息
Lombardo L, Carinci F, Martini M, Gemmati D, Nardone M, Siciliani G
机构信息
Postgraduate School of Orthodontics, University of Ferrara, Ferrara, Italy.
Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
出版信息
Oral Implantol (Rome). 2016 Nov 13;9(3):132-142. doi: 10.11138/orl/2016.9.3.132. eCollection 2016 Jul-Sep.
Abstract
PURPOSE
This study had the aim of comparing two different methods of analysing dentin sialoprotein (DSP) in the gingival crevicular fluid (GCF): the conventional eLISA approach and a new method involving the use of magnetic micro-beads coated with an antibody specific for DSP prior to eLISA analysis.
MATERIALS AND METHODS
GCF was taken from six patients following twelve weeks of orthodontic treatment using paper strips inserted into the mesial and distal sulci of the upper incisors, and analysed using both methods.
RESULTS
Statistical analysis of the results using the Mann-Whitney non-parametric test showed that the micro-bead approach conferred more reliability and less variability on the conventional eLISA approach. Furthermore, this method, for the first time, enables the quantification of the DSP in the sample in ng/μl.
CONCLUSIONS
The innovative micro-bead/eLISA approach proposed provides a reliable means of quantifying the DSP in the GCF.
摘要
目的
本研究旨在比较两种分析龈沟液（GCF）中牙本质涎蛋白（DSP）的不同方法：传统的酶联免疫吸附测定（eLISA）方法和一种新方法，即在eLISA分析之前使用包被有DSP特异性抗体的磁性微珠。
材料与方法
在12周正畸治疗后，从6名患者中采集龈沟液，使用插入上颌中切牙近中沟和远中沟的纸条采集，并用两种方法进行分析。
结果
使用曼-惠特尼非参数检验对结果进行统计分析表明，微珠法比传统的eLISA方法具有更高的可靠性和更低的变异性。此外，该方法首次能够以纳克/微升为单位对样品中的DSP进行定量。
结论
所提出的创新型微珠/eLISA方法为定量龈沟液中的DSP提供了一种可靠的手段。
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