Baslé M F, Mauras Y, Audran M, Clochon P, Rebel A, Allain P
Laboratory of Histology and Embryology, Faculty of Medicine, Angers, France.
J Bone Miner Res. 1990 Jan;5(1):41-7. doi: 10.1002/jbmr.5650050108.
In aging and in osteoporosis, decreased bone density is associated with decreased bone mass. However, changes in the bone mineral phase remain a matter for investigation. In particular, it is unknown whether bone mineral loss is directly related to reduction in bone mass or associated with changes in the concentration of mineral elements in mineralized bone tissue. In this study, the cortical bone concentration of elements was determined in biopsies of the ilium from 33 subjects (12 controls and 21 individuals with untreated severe osteoporosis). Calcium and phosphorus concentrations were evaluated in cortical and trabecular bone using energy-dispersive x-ray (EDX) microanalysis and inductively coupled plasma optical emission spectrometry (ICPOES). Bone concentrations of Na, K, Mg, Cu, Zn, Fe, Sr, Al, B, and Si were also determined in cortical bone using ICPOES. Additionally, the concentration of F in cortical bone was measured with a specific ion electrode and the concentration of Pb was determined by atomic absorption spectrometry. In mineralized bone tissue there was no significant age-dependent variation in the concentration of Ca, P, or other elements either in controls or in osteoporotic subjects. Moreover, the concentration of elements in bone tissue did not differ in the two groups. These results suggest that the decrease in bone density in osteoporosis is directly related to evolution of the bone mass, without detectable changes in the concentration of elements in bone.
在衰老和骨质疏松症中，骨密度降低与骨量减少相关。然而，骨矿物质相的变化仍是一个有待研究的问题。特别是，骨矿物质流失是否与骨量减少直接相关，或者是否与矿化骨组织中矿物质元素浓度的变化有关尚不清楚。在本研究中，测定了33名受试者（12名对照者和21名未经治疗的严重骨质疏松症患者）髂骨活检组织中皮质骨的元素浓度。使用能量色散X射线（EDX）微分析和电感耦合等离子体发射光谱法（ICPOES）评估皮质骨和小梁骨中的钙和磷浓度。还使用ICPOES测定了皮质骨中Na、K、Mg、Cu、Zn、Fe、Sr、Al、B和Si的骨浓度。此外，用特定离子电极测量皮质骨中的氟浓度，并用原子吸收光谱法测定铅浓度。在矿化骨组织中，无论是对照者还是骨质疏松症患者，Ca、P或其他元素的浓度均无明显的年龄依赖性变化。此外，两组骨组织中的元素浓度没有差异。这些结果表明，骨质疏松症中骨密度的降低与骨量的变化直接相关，而骨中元素浓度没有可检测到的变化。
