Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110 021 India.
Indian J Microbiol. 2007 Jun;47(2):144-52. doi: 10.1007/s12088-007-0028-4. Epub 2007 Jul 8.
Response surface methodology employing central composite design (CCD) was used to optimize fermentation medium for the production of cellulase-free, alkaline xylanase from Streptomyces violaceoruber under submerged fermentation. The design was employed by selecting wheat bran, peptone, beef extract, incubation time and agitation as model factors. A second-order quadratic model and response surface method showed that the optimum conditions for xylanase production (wheat bran 3.5 % (w/v), peptone 0.8 % (w/v), beef extract 0.8 % (w/v), incubation time 36 h and agitation 250 rpm) results in 3.0-fold improvement in alkaline xylanase production (1500.0 IUml(-1)) as compared to initial level (500.0 IUml(-1)) after 36 h of fermentation, whereas its value predicted by the quadratic model was 1347 IUml(-1). Analysis of variance (ANOVA) showed a high coefficient of determination (R(2)) value of 0.9718, ensuring a satisfactory adjustment of the quadratic model with the experimental data.The economical and cellulase-free nature of xylanase would enhance its applicability in pulp and paper industry.
采用中心组合设计（CCD）的响应面法被用于优化发酵培养基，以在浸没发酵条件下从紫色链霉菌中生产无纤维素、碱性木聚糖酶。该设计通过选择麦麸、蛋白胨、牛肉浸膏、培养时间和搅拌作为模型因素来进行。二次二次模型和响应面法表明，木聚糖酶生产的最佳条件（麦麸 3.5%（w/v）、蛋白胨 0.8%（w/v）、牛肉浸膏 0.8%（w/v）、培养时间 36 h 和搅拌 250 rpm）可使碱性木聚糖酶的产量（1500.0 IUml(-1)) 比初始水平（500.0 IUml(-1)) 提高 3 倍，发酵 36 h 后，而二次模型预测的值为 1347 IUml(-1)。方差分析（ANOVA）显示，二次模型与实验数据具有较高的决定系数（R(2)) 值为 0.9718，确保了对二次模型的满意调整。木聚糖酶的经济和无纤维素特性将增强其在制浆造纸工业中的适用性。
