对睡茄叶斑病及其对次生代谢物影响的研究。
Studies on leaf spot disease of Withania somnifera and its impact on secondary metabolites.
机构信息
Department of Biotechnology, Guru Nanak Dev University, Amritsar, 143 005 India.
出版信息
Indian J Microbiol. 2008 Dec;48(4):432-7. doi: 10.1007/s12088-008-0053-y. Epub 2009 Jan 8.
Abstract
During an investigation of the disease profile of Withania somnifera, it was observed that leaf spot is the most prevalent disease. Repeated isolations from infected leaf tissues and pathogenicity tests showed the association of fungal pathogen identified as Alternaria alternata (Fr.) Keissler. Scanning electron microscopy showed various histological changes in the leaf tissues of infected plants. A decrease in total content of reducing sugars (20%) and chlorophyll (26.5%) was observed in diseased leaves whereas an increase was noticed in proline (25%), free amino acids (3%) and proteins (74.3%). High performance thin layer chromatography (HPTLC) analysis of secondary metabolites viz. withanolides, withaferin-A and total alkaloids of the diseased leaves vis-à-vis control revealed reduction in withaferin-A and withanolides contents by 15.4% and 76.3% respectively, in contrast to an increase in total alkaloids by 49.3%, information hitherto unreported in W. somnifera.
摘要
在对睡茄疾病谱的调查中，观察到叶斑病是最普遍的疾病。从感染的叶片组织中反复分离和致病性试验表明，与鉴定为交替镰刀菌（Alternaria alternata（Fr.）Keissler）的真菌病原体有关。扫描电子显微镜显示，感染植株叶片组织有各种组织学变化。在患病叶片中，还原糖（20%）和叶绿素（26.5%）总含量下降，而脯氨酸（25%）、游离氨基酸（3%）和蛋白质（74.3%）增加。对次级代谢产物如醉茄内酯、醉茄素 A 和总生物碱的高效薄层色谱（HPTLC）分析表明，与对照相比，患病叶片中的醉茄素 A 和醉茄内酯含量分别下降了 15.4%和 76.3%，而总生物碱含量增加了 49.3%，这是在睡茄中尚未报道的信息。
相似文献
1
Studies on leaf spot disease of Withania somnifera and its impact on secondary metabolites.对睡茄叶斑病及其对次生代谢物影响的研究。
Indian J Microbiol. 2008 Dec;48(4):432-7. doi: 10.1007/s12088-008-0053-y. Epub 2009 Jan 8.
2
Leaf spot disease adversely affects human health-promoting constituents and withanolide biosynthesis in Withania somnifera (L.) Dunal.叶斑病对睡茄中促进人类健康的成分和睡茄内酯的生物合成产生不利影响。
J Appl Microbiol. 2017 Jan;122(1):153-165. doi: 10.1111/jam.13314. Epub 2016 Nov 15.
3
Endophyte-Mediated Modulation of Defense-Related Genes and Systemic Resistance in Withania somnifera (L.) Dunal under Alternaria alternata Stress.内生真菌介导的防御相关基因调控与系统性抗性在长柄木麻黄（L.）Dunal 下Alternaria alternata 胁迫
Appl Environ Microbiol. 2018 Apr 2;84(8). doi: 10.1128/AEM.02845-17. Print 2018 Apr 15.
4
Bacterial endophytes modulates the withanolide biosynthetic pathway and physiological performance in Withania somnifera under biotic stress.内生细菌调控生物胁迫下睡茄中醉茄内酯的生物合成途径和生理表现。
Microbiol Res. 2018 Jul-Aug;212-213:17-28. doi: 10.1016/j.micres.2018.04.006. Epub 2018 Apr 22.
5
First Report of Leaf Spot Disease in Withania coagulans Caused by Alternaria alternata in India.印度链格孢引起的凝固茄叶斑病的首次报道
Plant Dis. 2013 Mar;97(3):420. doi: 10.1094/PDIS-06-12-0559-PDN.
6
A validated HPTLC method for the simultaneous quantifications of three phenolic acids and three withanolides from Withania somnifera plants and its herbal products.一种经验证的 HPTLC 方法，用于同时定量测定来自睡茄植物及其草药产品的三种酚酸和三种甾体生物碱。
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Aug 15;1124:154-160. doi: 10.1016/j.jchromb.2019.06.009. Epub 2019 Jun 6.
7
Dynamics of reactive oxygen species and lignin biosynthesis during leaf spot disease of Withania somnifera (L.) Dunal.在睡茄（Withania somnifera（L.）Dunal.）叶斑病期间活性氧和木质素生物合成的动态。
Plant Biol (Stuttg). 2023 Aug;25(5):757-770. doi: 10.1111/plb.13548. Epub 2023 Jun 16.
8
Distribution of withaferin A, an anticancer potential agent, in different parts of two varieties of Withania somnifera (L.) Dunal. grown in Sri Lanka.抗癌潜在药物睡茄内酯A在斯里兰卡种植的两种睡茄（Withania somnifera (L.) Dunal）不同部位的分布情况。
Pak J Biol Sci. 2013 Feb 1;16(3):141-4. doi: 10.3923/pjbs.2013.141.144.
9
Metabolic characterization of Withania somnifera from different regions of India using NMR spectroscopy.采用 NMR 光谱法对来自印度不同地区的睡茄进行代谢特征分析。
Planta Med. 2011 Nov;77(17):1958-64. doi: 10.1055/s-0031-1279997. Epub 2011 Jul 4.
10
Hypoglycemic activity of withanolides and elicitated Withania somnifera.睡茄内酯及诱导型睡茄的降血糖活性。
Phytochemistry. 2015 Aug;116:283-289. doi: 10.1016/j.phytochem.2015.02.029. Epub 2015 Mar 18.
引用本文的文献
1
Phytochemical analysis of baby corn silk extracts.嫩玉米须提取物的植物化学分析。
J Ayurveda Integr Med. 2020 Jul-Sep;11(3):344-351. doi: 10.1016/j.jaim.2019.10.005. Epub 2020 Mar 10.
2
Wheat Straw Return Influences Nitrogen-Cycling and Pathogen Associated Soil Microbiota in a Wheat-Soybean Rotation System.小麦秸秆还田对小麦-大豆轮作系统中氮循环及与病原菌相关的土壤微生物群有影响。
Front Microbiol. 2019 Aug 8;10:1811. doi: 10.3389/fmicb.2019.01811. eCollection 2019.
3
Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice.睡茄对双酚 A 诱导的认知功能障碍和氧化应激小鼠的神经保护作用。
Behav Brain Funct. 2019 May 7;15(1):9. doi: 10.1186/s12993-019-0160-4.
4
Influence of phytochemical composition on antioxidant and reducing activities of Indian ginseng [ (L.) Dunal] root extracts.植物化学成分对印度人参[（L.）Dunal]根提取物抗氧化和还原活性的影响。
J Ginseng Res. 2018 Oct;42(4):463-469. doi: 10.1016/j.jgr.2017.05.002. Epub 2017 May 18.
5
Endophyte-Mediated Modulation of Defense-Related Genes and Systemic Resistance in Withania somnifera (L.) Dunal under Alternaria alternata Stress.内生真菌介导的防御相关基因调控与系统性抗性在长柄木麻黄（L.）Dunal 下Alternaria alternata 胁迫
Appl Environ Microbiol. 2018 Apr 2;84(8). doi: 10.1128/AEM.02845-17. Print 2018 Apr 15.
6
Addressing Challenges to Enhance the Bioactives of through Organ, Tissue, and Cell Culture Based Approaches.通过基于器官、组织和细胞培养的方法应对挑战以提高生物活性物质。
Biomed Res Int. 2017;2017:3278494. doi: 10.1155/2017/3278494. Epub 2017 Feb 16.
7
Evaluation and selection of candidate reference genes for normalization of quantitative RT-PCR in Withania somnifera (L.) Dunal.用于印度人参定量逆转录聚合酶链反应标准化的候选参考基因的评估与选择
PLoS One. 2015 Mar 13;10(3):e0118860. doi: 10.1371/journal.pone.0118860. eCollection 2015.
8
Carbon-Mercaptooctadecane/Carboxylated Multi-walled Carbon Nanotubes Composite Based Genosensor for Detection of Bacterial Meningitis.基于碳-巯基十八烷/羧基化多壁碳纳米管复合材料的基因传感器用于检测细菌性脑膜炎。
Indian J Microbiol. 2014 Jun;54(2):170-7. doi: 10.1007/s12088-013-0435-7. Epub 2013 Oct 27.
9
Influence of plant growth regulators on callus mediated regeneration and secondary metabolites synthesis in Withania somnifera (L.) Dunal.植物生长调节剂对睡茄（L.）Dunal 愈伤组织介导的再生和次生代谢物合成的影响。
Physiol Mol Biol Plants. 2013 Jan;19(1):117-25. doi: 10.1007/s12298-012-0146-2.
10
First record of the carmine spider mite, Tetranychus urticae, infesting Withania somnifera in India.首次记录在印度寄生睡茄的朱砂叶螨，Tetranychus urticae。
J Insect Sci. 2012;12:50. doi: 10.1673/031.012.5001.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Chemical studies of the leaves of Withania somnifera.睡茄叶的化学研究。
J Pharm Sci. 1961 Oct;50:876-7. doi: 10.1002/jps.2600501019.
3
Ultrastructure of the host-pathogen interface in daylily leaves infected by the rust fungus Puccinia hemerocallidis.萱草锈病菌（Puccinia hemerocallidis）侵染的萱草叶片中宿主-病原体界面的超微结构
Protoplasma. 2002 May;219(3-4):221-6. doi: 10.1007/s007090200023.
4
Microorganisms asssociated with Withania somnifera leaves.
Microbiol Res. 2001;156(4):303-9. doi: 10.1078/0944-5013-00094.
5
The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida.不同生物和非生物诱导子对白花曼陀罗毛状根培养物中托烷生物碱产量及成分的影响。
Enzyme Microb Technol. 2000 Feb 1;26(2-4):252-258. doi: 10.1016/s0141-0229(99)00137-4.
6
Withania somnifera Dunal (Ashwagandha): potential plant source of a promising drug for cancer chemotherapy and radiosensitization.南非醉茄（Ashwagandha）：癌症化疗和放射增敏有前景药物的潜在植物来源。
Indian J Exp Biol. 1996 Oct;34(10):927-32.
Zotero 插件